The dendritic cell (DC) specific pathogen-recognition receptor DCSIGN binds and internalizes antigens for degradation. The organization of DC-SIGN in microdomains is crucial for the binding and the internalization of virus particles, suggesting that these multimolecular assemblies act as docking site for pathogens like HIV-1 to invade the host. We have recently shown that DC-SIGN potentially associates with lipid rafts  and clathrin coated pits . Nevertheless, the nano-scale organization of DC-SIGN with respect to these lipid domains remains largely unresolved. To map the nanolandscape distribution of DC-SIGN on DC cell membranes we are exploiting state-of-the-art microscopic imaging techniques. Single fluorescent molecule detection together with multicolor labeling offers the possibility to elucidate organization and co-localization at <100 nm spatial resolution. Currently we are investigating the potential association of DC-SIGN with lipid rafts using a near-field optical microscope working under physiological conditions. Additionally we are planning a three color experiment to resolve the nano-landscape of DC-SIGN, lipid rafts and clathrin coated pits before and during endocytosis. The possible change in organization and association of DC-SIGN is essential for the understanding of the antigen uptake mechanism(s) from the membrane of DCs.  A. Cambi, et al., Journal of Cell Biology, 164, 145 (2004).  A. Cambi, et al., Nanoletters, In Press.
|Journal||European biophysics journal|
|Publication status||Published - 2007|
|Event||6th European Biophysics Congress, EBSA 2007 - London, United Kingdom|
Duration: 14 Jul 2007 → 19 Jul 2007
Conference number: 6