Mapping of enzymatic kinetics on a chip

Hoon Suk Rho, Han Gardeniers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherThe Chemical and Biological Microsystems Society
Pages1211-1213
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015
Conference number: 19
http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated titleMicroTas 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15
Internet address

Keywords

  • Concentration gradient
  • Enzyme
  • Microfluidics

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