Abstract
We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.
Original language | English |
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Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 1211-1213 |
Number of pages | 3 |
ISBN (Electronic) | 9780979806483 |
Publication status | Published - 2015 |
Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf |
Conference
Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 |
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Abbreviated title | MicroTas 2015 |
Country/Territory | Korea, Republic of |
City | Gyeongju |
Period | 25/10/15 → 29/10/15 |
Internet address |
Keywords
- Concentration gradient
- Enzyme
- Microfluidics