Mapping of enzymatic kinetics on a chip

Hoon Suk Rho; Han Gardeniers

Mapping of enzymatic kinetics on a chip

Hoon Suk Rho
, Han Gardeniers^*

^*Corresponding author for this work

Mesoscale Chemical Systems

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H₂O₂) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.

Original language	English
Title of host publication	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	The Chemical and Biological Microsystems Society
Pages	1211-1213
Number of pages	3
ISBN (Electronic)	9780979806483
Publication status	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTas 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15
Internet address	http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Keywords

Concentration gradient
Enzyme
Microfluidics

Cite this

@inproceedings{cd4cf2c8bc494ab0955208af2c51a865,

title = "Mapping of enzymatic kinetics on a chip",

abstract = "We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.",

keywords = "Concentration gradient, Enzyme, Microfluidics",

author = "Rho, \{Hoon Suk\} and Han Gardeniers",

year = "2015",

language = "English",

pages = "1211--1213",

booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "The Chemical and Biological Microsystems Society",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, MicroTas 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

url = "http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf",

}

Rho, HS & Gardeniers, H 2015, Mapping of enzymatic kinetics on a chip. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 1211-1213, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

TY - GEN

T1 - Mapping of enzymatic kinetics on a chip

AU - Rho, Hoon Suk

AU - Gardeniers, Han

N1 - Conference code: 19

PY - 2015

Y1 - 2015

N2 - We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.

AB - We show the development of a microfluidic platform that is capable of performing 36 parallel reactions with 36 combinations of the concentrations of two reagents. With the device, we obtained a 3D enzymatic reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2) in a nanoliter scale volume. The system could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other.

KW - Concentration gradient

KW - Enzyme

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/84983266605

M3 - Conference contribution

AN - SCOPUS:84983266605

SP - 1211

EP - 1213

BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - The Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

Mapping of enzymatic kinetics on a chip

Abstract

Conference

Keywords

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