Measurements on Single DNA Molecules

B.G. de Grooth; A. Agronskaia; M.L. Bennink; S.J.T. van Noort; K.O. van der Werf; J. Greve

doi:10.1007/978-94-011-5010-1_7

Measurements on Single DNA Molecules

B.G. de Grooth, A. Agronskaia, M.L. Bennink, S.J.T. van Noort, K.O. van der Werf, J. Greve

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.

Original language	English
Title of host publication	Sensor technology in the Netherlands - state of the art
Subtitle of host publication	proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands
Editors	A. van den Berg, P. Bergveld
Place of Publication	Dordrecht
Publisher	Kluwer Academic Publishers
Pages	43-48
Number of pages	6
ISBN (Electronic)	978-94-011-5010-1
ISBN (Print)	978-94-010-6103-2
DOIs	https://doi.org/10.1007/978-94-011-5010-1_7
Publication status	Published - 1 Mar 1998
Event	Dutch Sensor Conference 1998: Sensor Technology in the Netherlands: State of the Art - University of Twente, Enschede, Netherlands Duration: 2 Mar 1998 → 3 Mar 1998

Publication series

Name	MESA monographs
Publisher	Kluwer Academic Publishers
Volume	2

Conference

Conference	Dutch Sensor Conference 1998
Country	Netherlands
City	Enschede
Period	2/03/98 → 3/03/98

Keywords

Optical tweezers
DNA overstretching
Denaturation
AFM
Photolyase

Access to Document

10.1007/978-94-011-5010-1_7

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de Grooth, B. G., Agronskaia, A., Bennink, M. L., van Noort, S. J. T., van der Werf, K. O., & Greve, J. (1998). Measurements on Single DNA Molecules. In A. van den Berg, & P. Bergveld (Eds.), Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands (pp. 43-48). (MESA monographs; Vol. 2). Dordrecht: Kluwer Academic Publishers. https://doi.org/10.1007/978-94-011-5010-1_7

de Grooth, B.G. ; Agronskaia, A. ; Bennink, M.L. ; van Noort, S.J.T. ; van der Werf, K.O. ; Greve, J. / Measurements on Single DNA Molecules. Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. editor / A. van den Berg ; P. Bergveld. Dordrecht : Kluwer Academic Publishers, 1998. pp. 43-48 (MESA monographs).

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title = "Measurements on Single DNA Molecules",

abstract = "All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.",

keywords = "Optical tweezers, DNA overstretching, Denaturation, AFM, Photolyase",

author = "{de Grooth}, B.G. and A. Agronskaia and M.L. Bennink and {van Noort}, S.J.T. and {van der Werf}, K.O. and J. Greve",

year = "1998",

month = "3",

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doi = "10.1007/978-94-011-5010-1_7",

language = "English",

isbn = "978-94-010-6103-2",

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de Grooth, BG, Agronskaia, A, Bennink, ML, van Noort, SJT, van der Werf, KO & Greve, J 1998, Measurements on Single DNA Molecules. in A van den Berg & P Bergveld (eds), Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. MESA monographs, vol. 2, Kluwer Academic Publishers, Dordrecht, pp. 43-48, Dutch Sensor Conference 1998, Enschede, Netherlands, 2/03/98. https://doi.org/10.1007/978-94-011-5010-1_7

Measurements on Single DNA Molecules. / de Grooth, B.G.; Agronskaia, A.; Bennink, M.L.; van Noort, S.J.T.; van der Werf, K.O.; Greve, J.

Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. ed. / A. van den Berg; P. Bergveld. Dordrecht : Kluwer Academic Publishers, 1998. p. 43-48 (MESA monographs; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AU - Greve, J.

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N2 - All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.

AB - All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.

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KW - DNA overstretching

KW - Denaturation

KW - AFM

KW - Photolyase

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de Grooth BG, Agronskaia A, Bennink ML, van Noort SJT, van der Werf KO, Greve J. Measurements on Single DNA Molecules. In van den Berg A, Bergveld P, editors, Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. Dordrecht: Kluwer Academic Publishers. 1998. p. 43-48. (MESA monographs). https://doi.org/10.1007/978-94-011-5010-1_7