All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.
|Publisher||Kluwer Academic Publishers|
|Conference||Dutch Sensor Conference 1998|
|Period||2/03/98 → 3/03/98|
- Optical tweezers
- DNA overstretching