Measurements on Single DNA Molecules

B.G. de Grooth, A. Agronskaia, M.L. Bennink, S.J.T. van Noort, K.O. van der Werf, J. Greve

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    All our understanding of how DNA works is based on evidence obtained with bulk methods. Moreover, information on the detailed conformation of DNA and interactions between DNA and proteins at a high resolution, have been obtained almost entirely under highly nonphysiological conditions (vacuum, low temperature etc.). Recently several methods have become available that make it possible to study individual bio-molecules under physiological conditions. We will describe three of these methods and there application to DNA research: flow analysis of individual molecules, measuring mechanical forces of bio-molecules using optical tweezers and using an AFM to study the dynamics of DNA and DNA protein interactions.
    Original languageEnglish
    Title of host publicationSensor technology in the Netherlands - state of the art
    Subtitle of host publicationproceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands
    EditorsA. van den Berg, P. Bergveld
    Place of PublicationDordrecht
    PublisherKluwer Academic Publishers
    Pages43-48
    Number of pages6
    ISBN (Electronic)978-94-011-5010-1
    ISBN (Print)978-94-010-6103-2
    DOIs
    Publication statusPublished - 1 Mar 1998
    EventDutch Sensor Conference 1998: Sensor Technology in the Netherlands: State of the Art - University of Twente, Enschede, Netherlands
    Duration: 2 Mar 19983 Mar 1998

    Publication series

    NameMESA monographs
    PublisherKluwer Academic Publishers
    Volume2

    Conference

    ConferenceDutch Sensor Conference 1998
    CountryNetherlands
    CityEnschede
    Period2/03/983/03/98

    Keywords

    • Optical tweezers
    • DNA overstretching
    • Denaturation
    • AFM
    • Photolyase

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  • Cite this

    de Grooth, B. G., Agronskaia, A., Bennink, M. L., van Noort, S. J. T., van der Werf, K. O., & Greve, J. (1998). Measurements on Single DNA Molecules. In A. van den Berg, & P. Bergveld (Eds.), Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands (pp. 43-48). (MESA monographs; Vol. 2). Dordrecht: Kluwer Academic Publishers. https://doi.org/10.1007/978-94-011-5010-1_7