Measuring barrier function in a gut-on-chip

Elsbeth G.B.M. Bossink, Mariia Zakharova, Mathieu Odijk, Loes Segerink

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Here, we present a novel, cleanroom-free way of integrating electrodes in a typical poly(dimethylsiloxane) (PDMS) organ-on-chip (OoC) for real-time measurement of the barrier function during cell culture utilizing impedance spectroscopy. With the proposed configuration, it is possible to multiplex both the electrodes and the OoCs in one device. To illustrate the applicability of the new method, a PDMS device with three parallel OoC units was fabricated and lined with Caco-2 cells mimicking a gut-on-chip. We monitored and observed the formation, as well as disruption of the cell barrier during cell culture.
Original languageEnglish
Title of host publicationμTAS 2020
Subtitle of host publicationThe 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages943-944
Number of pages2
Publication statusPublished - 21 Sep 2020

Keywords

  • Transepithelial electrical resistance
  • Impedance Spectroscopy
  • Electrode integration
  • Gut-on-a-chip

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