Measuring barrier function in a gut-on-chip

Elsbeth G.B.M. Bossink; Mariia  Zakharova; Mathieu  Odijk; Loes Segerink

Measuring barrier function in a gut-on-chip

Elsbeth G.B.M. Bossink, Mariia Zakharova, Mathieu Odijk, Loes Segerink

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Here, we present a novel, cleanroom-free way of integrating electrodes in a typical poly(dimethylsiloxane) (PDMS) organ-on-chip (OoC) for real-time measurement of the barrier function during cell culture utilizing impedance spectroscopy. With the proposed configuration, it is possible to multiplex both the electrodes and the OoCs in one device. To illustrate the applicability of the new method, a PDMS device with three parallel OoC units was fabricated and lined with Caco-2 cells mimicking a gut-on-chip. We monitored and observed the formation, as well as disruption of the cell barrier during cell culture.

Original language	English
Title of host publication	μTAS 2020
Subtitle of host publication	The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages	943-944
Number of pages	2
Publication status	Published - 21 Sept 2020

Keywords

Transepithelial electrical resistance
Impedance Spectroscopy
Electrode integration
Gut-on-a-chip

Cite this

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title = "Measuring barrier function in a gut-on-chip",

abstract = "Here, we present a novel, cleanroom-free way of integrating electrodes in a typical poly(dimethylsiloxane) (PDMS) organ-on-chip (OoC) for real-time measurement of the barrier function during cell culture utilizing impedance spectroscopy. With the proposed configuration, it is possible to multiplex both the electrodes and the OoCs in one device. To illustrate the applicability of the new method, a PDMS device with three parallel OoC units was fabricated and lined with Caco-2 cells mimicking a gut-on-chip. We monitored and observed the formation, as well as disruption of the cell barrier during cell culture.",

keywords = "Transepithelial electrical resistance, Impedance Spectroscopy, Electrode integration, Gut-on-a-chip",

author = "Bossink, {Elsbeth G.B.M.} and Mariia Zakharova and Mathieu Odijk and Loes Segerink",

year = "2020",

month = sep,

day = "21",

language = "English",

isbn = "978-1-7334190-1-7",

pages = "943--944",

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TY - GEN

T1 - Measuring barrier function in a gut-on-chip

AU - Bossink, Elsbeth G.B.M.

AU - Zakharova, Mariia

AU - Odijk, Mathieu

AU - Segerink, Loes

PY - 2020/9/21

Y1 - 2020/9/21

N2 - Here, we present a novel, cleanroom-free way of integrating electrodes in a typical poly(dimethylsiloxane) (PDMS) organ-on-chip (OoC) for real-time measurement of the barrier function during cell culture utilizing impedance spectroscopy. With the proposed configuration, it is possible to multiplex both the electrodes and the OoCs in one device. To illustrate the applicability of the new method, a PDMS device with three parallel OoC units was fabricated and lined with Caco-2 cells mimicking a gut-on-chip. We monitored and observed the formation, as well as disruption of the cell barrier during cell culture.

AB - Here, we present a novel, cleanroom-free way of integrating electrodes in a typical poly(dimethylsiloxane) (PDMS) organ-on-chip (OoC) for real-time measurement of the barrier function during cell culture utilizing impedance spectroscopy. With the proposed configuration, it is possible to multiplex both the electrodes and the OoCs in one device. To illustrate the applicability of the new method, a PDMS device with three parallel OoC units was fabricated and lined with Caco-2 cells mimicking a gut-on-chip. We monitored and observed the formation, as well as disruption of the cell barrier during cell culture.

KW - Transepithelial electrical resistance

KW - Impedance Spectroscopy

KW - Electrode integration

KW - Gut-on-a-chip

M3 - Conference contribution

SN - 978-1-7334190-1-7

SP - 943

EP - 944

BT - μTAS 2020

ER -

Measuring barrier function in a gut-on-chip

Abstract

Keywords

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