TY - JOUR
T1 - Measuring both pH and O2 with a single on-chip sensor in cultures of human pluripotent stem cell-derived cardiomyocytes to track induced changes in cellular metabolism
AU - Tanumihardja, Esther
AU - Slaats, Rolf H.
AU - van der Meer, Andries D.
AU - Passier, Robert
AU - Olthuis, Wouter
AU - van den Berg, Albert
N1 - ACS deal
Funding Information:
This research was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 669768, VESCEL project).
Publisher Copyright:
© 2020 American Chemical Society.
PY - 2021/1/22
Y1 - 2021/1/22
N2 - In vitro studies which focus on cellular metabolism can benefit from time-resolved readouts from the living cells. pH and O2 concentration are fundamental parameters upon which cellular metabolism is often inferred. This work demonstrates a novel use of a ruthenium oxide (RuOx) electrode for in vitro studies. The RuOx electrode was characterized to measure both pH and O2 using two different modes. When operated potentiometrically, continuous pH reading can be obtained, and O2 concentration can be measured chronoamperometrically. In this work, we demonstrate the use of the RuOx electrodes in inferring two different types of metabolism of human pluripotent stem cell-derived cardiomyocytes. We also show and discuss the interpretation of the measurements into meaningful extracellular acidification rates and oxygen consumption rates of the cells. Overall, we present the RuOx electrode as a versatile and powerful tool in in vitro cell metabolism studies, especially in comparative settings.
AB - In vitro studies which focus on cellular metabolism can benefit from time-resolved readouts from the living cells. pH and O2 concentration are fundamental parameters upon which cellular metabolism is often inferred. This work demonstrates a novel use of a ruthenium oxide (RuOx) electrode for in vitro studies. The RuOx electrode was characterized to measure both pH and O2 using two different modes. When operated potentiometrically, continuous pH reading can be obtained, and O2 concentration can be measured chronoamperometrically. In this work, we demonstrate the use of the RuOx electrodes in inferring two different types of metabolism of human pluripotent stem cell-derived cardiomyocytes. We also show and discuss the interpretation of the measurements into meaningful extracellular acidification rates and oxygen consumption rates of the cells. Overall, we present the RuOx electrode as a versatile and powerful tool in in vitro cell metabolism studies, especially in comparative settings.
KW - UT-Hybrid-D
KW - Dual electrode
KW - Extracellular acidification rate
KW - Oxygen consumption rate
KW - Ruthenium oxide
KW - Cell metabolism
UR - http://www.scopus.com/inward/record.url?scp=85099179364&partnerID=8YFLogxK
U2 - 10.1021/acssensors.0c02282
DO - 10.1021/acssensors.0c02282
M3 - Article
AN - SCOPUS:85099179364
SN - 2379-3694
VL - 6
SP - 267
EP - 274
JO - ACS sensors
JF - ACS sensors
IS - 1
ER -