Measuring the intravitreal mobility of nanomedicines with singe-particle tracking microscopy

T.F. Martens, D. Vercauteren, K. Forier, H. Deschout, K. Remaut, R. Paesen, M. Ameloot, Johannes F.J. Engbersen, J. Demeester, S.C. de Smedt, K. Braeckmans

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration.
Original languageEnglish
Pages (from-to)1955-1968
Number of pages14
JournalNanomedicine : nanotechnology, biology and medicine
Volume8
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • IR-88374
  • METIS-299732

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