Mechanical properties of single electrospun collagen type I fibers

Lanti Yang, Carel F.C. Fitie, Kees van der Werf, Martin L. Bennink, Pieter J. Dijkstra, Jan Feijen

Research output: Contribution to journalArticleAcademicpeer-review

175 Citations (Scopus)

Abstract

The mechanical properties of single electrospun collagen fibers were investigated using scanning mode bending tests performed with an AFM. Electrospun collagen fibers with diameters ranging from 100 to 600 nm were successfully produced by electrospinning of an 8% w/v solution of acid soluble collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP). Circular dichroism (CD) spectroscopy showed that 45% of the triple helical structure of collagen molecules was denatured in the electrospun fibers. The electrospun fibers were water soluble and became insoluble after cross-linking with glutaraldehyde vapor for 24 h. The bending moduli and shear moduli of both non- and cross-linked single electrospun collagen fibers were determined by scanning mode bending tests after depositing the fibers on glass substrates containing micro-channels. The bending moduli of the electrospun fibers ranged from 1.3 to 7.8 GPa at ambient conditions and ranged from 0.07 to 0.26 MPa when immersed in PBS buffer. As the diameter of the fibrils increased, a decrease in bending modulus was measured clearly indicating mechanical anisotropy of the fiber. Cross-linking of the electrospun fibers with glutaraldehyde vapor increased the shear modulus of the fiber from not, vert, similar30 to not, vert, similar50 MPa at ambient conditions.
Original languageUndefined
Pages (from-to)955-962
JournalBiomaterials
Volume29
Issue number8
DOIs
Publication statusPublished - 2008

Keywords

  • METIS-244917
  • IR-69417

Cite this

Yang, Lanti ; Fitie, Carel F.C. ; van der Werf, Kees ; Bennink, Martin L. ; Dijkstra, Pieter J. ; Feijen, Jan. / Mechanical properties of single electrospun collagen type I fibers. In: Biomaterials. 2008 ; Vol. 29, No. 8. pp. 955-962.
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abstract = "The mechanical properties of single electrospun collagen fibers were investigated using scanning mode bending tests performed with an AFM. Electrospun collagen fibers with diameters ranging from 100 to 600 nm were successfully produced by electrospinning of an 8{\%} w/v solution of acid soluble collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP). Circular dichroism (CD) spectroscopy showed that 45{\%} of the triple helical structure of collagen molecules was denatured in the electrospun fibers. The electrospun fibers were water soluble and became insoluble after cross-linking with glutaraldehyde vapor for 24 h. The bending moduli and shear moduli of both non- and cross-linked single electrospun collagen fibers were determined by scanning mode bending tests after depositing the fibers on glass substrates containing micro-channels. The bending moduli of the electrospun fibers ranged from 1.3 to 7.8 GPa at ambient conditions and ranged from 0.07 to 0.26 MPa when immersed in PBS buffer. As the diameter of the fibrils increased, a decrease in bending modulus was measured clearly indicating mechanical anisotropy of the fiber. Cross-linking of the electrospun fibers with glutaraldehyde vapor increased the shear modulus of the fiber from not, vert, similar30 to not, vert, similar50 MPa at ambient conditions.",
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Mechanical properties of single electrospun collagen type I fibers. / Yang, Lanti; Fitie, Carel F.C.; van der Werf, Kees; Bennink, Martin L.; Dijkstra, Pieter J.; Feijen, Jan.

In: Biomaterials, Vol. 29, No. 8, 2008, p. 955-962.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Mechanical properties of single electrospun collagen type I fibers

AU - Yang, Lanti

AU - Fitie, Carel F.C.

AU - van der Werf, Kees

AU - Bennink, Martin L.

AU - Dijkstra, Pieter J.

AU - Feijen, Jan

PY - 2008

Y1 - 2008

N2 - The mechanical properties of single electrospun collagen fibers were investigated using scanning mode bending tests performed with an AFM. Electrospun collagen fibers with diameters ranging from 100 to 600 nm were successfully produced by electrospinning of an 8% w/v solution of acid soluble collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP). Circular dichroism (CD) spectroscopy showed that 45% of the triple helical structure of collagen molecules was denatured in the electrospun fibers. The electrospun fibers were water soluble and became insoluble after cross-linking with glutaraldehyde vapor for 24 h. The bending moduli and shear moduli of both non- and cross-linked single electrospun collagen fibers were determined by scanning mode bending tests after depositing the fibers on glass substrates containing micro-channels. The bending moduli of the electrospun fibers ranged from 1.3 to 7.8 GPa at ambient conditions and ranged from 0.07 to 0.26 MPa when immersed in PBS buffer. As the diameter of the fibrils increased, a decrease in bending modulus was measured clearly indicating mechanical anisotropy of the fiber. Cross-linking of the electrospun fibers with glutaraldehyde vapor increased the shear modulus of the fiber from not, vert, similar30 to not, vert, similar50 MPa at ambient conditions.

AB - The mechanical properties of single electrospun collagen fibers were investigated using scanning mode bending tests performed with an AFM. Electrospun collagen fibers with diameters ranging from 100 to 600 nm were successfully produced by electrospinning of an 8% w/v solution of acid soluble collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP). Circular dichroism (CD) spectroscopy showed that 45% of the triple helical structure of collagen molecules was denatured in the electrospun fibers. The electrospun fibers were water soluble and became insoluble after cross-linking with glutaraldehyde vapor for 24 h. The bending moduli and shear moduli of both non- and cross-linked single electrospun collagen fibers were determined by scanning mode bending tests after depositing the fibers on glass substrates containing micro-channels. The bending moduli of the electrospun fibers ranged from 1.3 to 7.8 GPa at ambient conditions and ranged from 0.07 to 0.26 MPa when immersed in PBS buffer. As the diameter of the fibrils increased, a decrease in bending modulus was measured clearly indicating mechanical anisotropy of the fiber. Cross-linking of the electrospun fibers with glutaraldehyde vapor increased the shear modulus of the fiber from not, vert, similar30 to not, vert, similar50 MPa at ambient conditions.

KW - METIS-244917

KW - IR-69417

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