Membrane integration in biomedical microdevices

Magdalena Malankowska

Research output: ThesisPhD Thesis - Research UT, graduation externalAcademic

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Abstract

The target of the research presented in this thesis is a design, development and fabrication of a microfluidic device with integrated membrane in the form of a membrane contactor for various biological applications. The microfluidic devices are fabricated and tested for oxygenation of blood and separation of anaesthetic gas.

In the first part of the work, the microfluidic system for blood oxygenation, so called lung-on-a-chip, is introduced. In such system, one chamber is devoted to pure oxygen, and the other chamber is designed for blood and they are separated by a dense permeable membrane. Computer modelling is performed in order to design the liquid chamber with homogenous liquid flow, low pressure drop of the system and low shear stress without compensation of high oxygenation. Two different microdevice geometries are proposed: alveolar and meander type design with vertical membrane arrangement. Fabricated devices as well as integrated membranes are made of PDMS by soft-lithography and their surface is modified in order to make them more hydrophilic. The experiments of blood oxygenation are performed and the oxygen concentration is measured by an oximeter electrode and compared to the mathematically modelled values. The parameter sensitivity and the possible improvements of the proposed architectures based on the mathematical simulations are presented as well.

The second part of the thesis, introduces the concept of an alveolar microfluidic device as gas-ionic liquid micro-contactor for removal of CO2 from anaesthesia gas, containing Xe. The working principle involves the transport of CO2 through a flat PDMS membrane followed by the capture and enzymatic bioconversion in the ionic liquid solvent. As proof of concept demonstration, simple gas permeability experiments are performed followed by the experiments with ionic liquid and ionic liquid with the enzyme.

Finally, an alternative concept of a microfluidic device with an integrated membrane in the form of a fractal geometry with nanonozzles as pores at the vertices of the third-level octahedra for the controlled addition of gaseous species is introduced. Fractal geometry, that is a three-dimensional repetitive unit, is fabricated by a combination of anisotropic etching of silicon and corner lithography. As a proof of concept, simple gas permeation experiments are performed, and the achieved results are compared to the gas permeation obtained by a dense PDMS membrane.
Original languageEnglish
Awarding Institution
  • Universidad de Zaragoza
Supervisors/Advisors
  • Gardeniers, J.G.E., Supervisor
  • Pina, Maria Pilar, Supervisor
  • Coelhoso, I.M., Supervisor, External person
Award date12 Jan 2018
Place of PublicationZaragoza
Publisher
Publication statusPublished - 12 Jan 2018

Fingerprint

Membranes
Microfluidics
Oxygenation
Ionic liquids
Blood
Gases
Permeation
Fractals
Lithography
Geometry
Experiments
Oximeters
Anisotropic etching
Bioconversion
Anesthetics
Oxygen
Gas permeability
Liquids
Pressure drop
Shear stress

Cite this

Malankowska, M. (2018). Membrane integration in biomedical microdevices. Zaragoza: Universidad de Zaragoza.
Malankowska, Magdalena. / Membrane integration in biomedical microdevices. Zaragoza : Universidad de Zaragoza, 2018.
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title = "Membrane integration in biomedical microdevices",
abstract = "The target of the research presented in this thesis is a design, development and fabrication of a microfluidic device with integrated membrane in the form of a membrane contactor for various biological applications. The microfluidic devices are fabricated and tested for oxygenation of blood and separation of anaesthetic gas.In the first part of the work, the microfluidic system for blood oxygenation, so called lung-on-a-chip, is introduced. In such system, one chamber is devoted to pure oxygen, and the other chamber is designed for blood and they are separated by a dense permeable membrane. Computer modelling is performed in order to design the liquid chamber with homogenous liquid flow, low pressure drop of the system and low shear stress without compensation of high oxygenation. Two different microdevice geometries are proposed: alveolar and meander type design with vertical membrane arrangement. Fabricated devices as well as integrated membranes are made of PDMS by soft-lithography and their surface is modified in order to make them more hydrophilic. The experiments of blood oxygenation are performed and the oxygen concentration is measured by an oximeter electrode and compared to the mathematically modelled values. The parameter sensitivity and the possible improvements of the proposed architectures based on the mathematical simulations are presented as well.The second part of the thesis, introduces the concept of an alveolar microfluidic device as gas-ionic liquid micro-contactor for removal of CO2 from anaesthesia gas, containing Xe. The working principle involves the transport of CO2 through a flat PDMS membrane followed by the capture and enzymatic bioconversion in the ionic liquid solvent. As proof of concept demonstration, simple gas permeability experiments are performed followed by the experiments with ionic liquid and ionic liquid with the enzyme. Finally, an alternative concept of a microfluidic device with an integrated membrane in the form of a fractal geometry with nanonozzles as pores at the vertices of the third-level octahedra for the controlled addition of gaseous species is introduced. Fractal geometry, that is a three-dimensional repetitive unit, is fabricated by a combination of anisotropic etching of silicon and corner lithography. As a proof of concept, simple gas permeation experiments are performed, and the achieved results are compared to the gas permeation obtained by a dense PDMS membrane.",
author = "Magdalena Malankowska",
note = "Erasmus Mundus Doctorate in Membrane Engineering (EUDIME)",
year = "2018",
month = "1",
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language = "English",
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Malankowska, M 2018, 'Membrane integration in biomedical microdevices', Universidad de Zaragoza, Zaragoza.

Membrane integration in biomedical microdevices. / Malankowska, Magdalena.

Zaragoza : Universidad de Zaragoza, 2018.

Research output: ThesisPhD Thesis - Research UT, graduation externalAcademic

TY - THES

T1 - Membrane integration in biomedical microdevices

AU - Malankowska, Magdalena

N1 - Erasmus Mundus Doctorate in Membrane Engineering (EUDIME)

PY - 2018/1/12

Y1 - 2018/1/12

N2 - The target of the research presented in this thesis is a design, development and fabrication of a microfluidic device with integrated membrane in the form of a membrane contactor for various biological applications. The microfluidic devices are fabricated and tested for oxygenation of blood and separation of anaesthetic gas.In the first part of the work, the microfluidic system for blood oxygenation, so called lung-on-a-chip, is introduced. In such system, one chamber is devoted to pure oxygen, and the other chamber is designed for blood and they are separated by a dense permeable membrane. Computer modelling is performed in order to design the liquid chamber with homogenous liquid flow, low pressure drop of the system and low shear stress without compensation of high oxygenation. Two different microdevice geometries are proposed: alveolar and meander type design with vertical membrane arrangement. Fabricated devices as well as integrated membranes are made of PDMS by soft-lithography and their surface is modified in order to make them more hydrophilic. The experiments of blood oxygenation are performed and the oxygen concentration is measured by an oximeter electrode and compared to the mathematically modelled values. The parameter sensitivity and the possible improvements of the proposed architectures based on the mathematical simulations are presented as well.The second part of the thesis, introduces the concept of an alveolar microfluidic device as gas-ionic liquid micro-contactor for removal of CO2 from anaesthesia gas, containing Xe. The working principle involves the transport of CO2 through a flat PDMS membrane followed by the capture and enzymatic bioconversion in the ionic liquid solvent. As proof of concept demonstration, simple gas permeability experiments are performed followed by the experiments with ionic liquid and ionic liquid with the enzyme. Finally, an alternative concept of a microfluidic device with an integrated membrane in the form of a fractal geometry with nanonozzles as pores at the vertices of the third-level octahedra for the controlled addition of gaseous species is introduced. Fractal geometry, that is a three-dimensional repetitive unit, is fabricated by a combination of anisotropic etching of silicon and corner lithography. As a proof of concept, simple gas permeation experiments are performed, and the achieved results are compared to the gas permeation obtained by a dense PDMS membrane.

AB - The target of the research presented in this thesis is a design, development and fabrication of a microfluidic device with integrated membrane in the form of a membrane contactor for various biological applications. The microfluidic devices are fabricated and tested for oxygenation of blood and separation of anaesthetic gas.In the first part of the work, the microfluidic system for blood oxygenation, so called lung-on-a-chip, is introduced. In such system, one chamber is devoted to pure oxygen, and the other chamber is designed for blood and they are separated by a dense permeable membrane. Computer modelling is performed in order to design the liquid chamber with homogenous liquid flow, low pressure drop of the system and low shear stress without compensation of high oxygenation. Two different microdevice geometries are proposed: alveolar and meander type design with vertical membrane arrangement. Fabricated devices as well as integrated membranes are made of PDMS by soft-lithography and their surface is modified in order to make them more hydrophilic. The experiments of blood oxygenation are performed and the oxygen concentration is measured by an oximeter electrode and compared to the mathematically modelled values. The parameter sensitivity and the possible improvements of the proposed architectures based on the mathematical simulations are presented as well.The second part of the thesis, introduces the concept of an alveolar microfluidic device as gas-ionic liquid micro-contactor for removal of CO2 from anaesthesia gas, containing Xe. The working principle involves the transport of CO2 through a flat PDMS membrane followed by the capture and enzymatic bioconversion in the ionic liquid solvent. As proof of concept demonstration, simple gas permeability experiments are performed followed by the experiments with ionic liquid and ionic liquid with the enzyme. Finally, an alternative concept of a microfluidic device with an integrated membrane in the form of a fractal geometry with nanonozzles as pores at the vertices of the third-level octahedra for the controlled addition of gaseous species is introduced. Fractal geometry, that is a three-dimensional repetitive unit, is fabricated by a combination of anisotropic etching of silicon and corner lithography. As a proof of concept, simple gas permeation experiments are performed, and the achieved results are compared to the gas permeation obtained by a dense PDMS membrane.

M3 - PhD Thesis - Research UT, graduation external

PB - Universidad de Zaragoza

CY - Zaragoza

ER -

Malankowska M. Membrane integration in biomedical microdevices. Zaragoza: Universidad de Zaragoza, 2018.