Membrane interactions and fibrillization of alpha-synuclein play an essential role in membrane disruption

Himanshu Chaudhary, A. Stefanovic, Vinod Subramaniam, Mireille Maria Anna Elisabeth Claessens

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

We studied α-synuclein (αS) aggregation in giant vesicles, and observed dramatic membrane disintegration, as well as lipid incorporation into micrometer-sized suprafibrillar aggregates. In the presence of dye-filled vesicles, dye leakage and fibrillization happen concurrently. However, growing fibrils do not impair the integrity of phospholipid vesicles that have a low affinity for αS. Seeding αS aggregation accelerates dye leakage, indicating that oligomeric species are not required to explain the observed effect. The evolving picture suggests that fibrils that appear in solution bind membranes and recruit membrane-bound monomers, resulting in lipid extraction, membrane destabilization and the formation of lipid-containing suprafibrillar aggregates
Original languageUndefined
Pages (from-to)4457-4463
Number of pages7
JournalFEBS letters
Volume588
DOIs
Publication statusPublished - 2014

Keywords

  • METIS-306381
  • IR-94957

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