Methacrylated human recombinant collagen peptide as a hydrogel for manipulating and monitoring stiffness-related cardiac cell behavior

Dylan Mostert, Ignasi Jorba, Bart G.W. Groenen, Robert Passier, Marie José T.H. Goumans, Huibert A. van Boxtel, Nicholas A. Kurniawan, Carlijn V.C. Bouten*, Leda Klouda

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Downloads (Pure)

Abstract

Environmental stiffness is a crucial determinant of cell function. There is a long-standing quest for reproducible and (human matrix) bio-mimicking biomaterials with controllable mechanical properties to unravel the relationship between stiffness and cell behavior. Here, we evaluate methacrylated human recombinant collagen peptide (RCPhC1-MA) hydrogels as a matrix to control 3D microenvironmental stiffness and monitor cardiac cell response. We show that RCPhC1-MA can form hydrogels with reproducible stiffness in the range of human developmental and adult myocardium. Cardiomyocytes (hPSC-CMs) and cardiac fibroblasts (cFBs) remain viable for up to 14 days inside RCPhC1-MA hydrogels while the effect of hydrogel stiffness on extracellular matrix production and hPSC-CM contractility can be monitored in real-time. Interestingly, whereas the beating behavior of the hPSC-CM monocultures is affected by environmental stiffness, this effect ceases when cFBs are present. Together, we demonstrate RCPhC1-MA to be a promising candidate to mimic and control the 3D biomechanical environment of cardiac cells.

Original languageEnglish
Article number106423
JournaliScience
Volume26
Issue number4
DOIs
Publication statusPublished - 21 Apr 2023
Externally publishedYes

Keywords

  • Biomaterials
  • Cell biology
  • Materials in biotechnology
  • Stem cells research

Fingerprint

Dive into the research topics of 'Methacrylated human recombinant collagen peptide as a hydrogel for manipulating and monitoring stiffness-related cardiac cell behavior'. Together they form a unique fingerprint.

Cite this