Methodological guidelines to study extracellular vesicles

Frank A.W. Coumans, Alain R. Brisson, Edit I. Buzas, Françoise Dignat-George, Esther E.E. Drees, Samir El-Andaloussi, Costanza Emanueli, Aleksandra Gasecka, An Hendrix, Andrew F. Hill, Romaric Lacroix, Yi Lee, Ton G. Van Leeuwen, Nigel Mackman, Imre Mäger, John P. Nolan, Edwin Van Der Pol, D. Michiel Pegtel, Susmita Sahoo, Pia R.M. SiljanderGuus Sturk, Olivier De Wever, Rienk Nieuwland*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

703 Citations (Scopus)

Abstract

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

Original languageEnglish
Pages (from-to)1632-1648
Number of pages17
JournalCirculation research
Volume120
Issue number10
DOIs
Publication statusPublished - 12 May 2017
Externally publishedYes

Keywords

  • cardiovascular diseases
  • exosomes
  • extracellular vesicles
  • methods
  • reference standards
  • n/a OA procedure

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