Methods and instrumentation for quantitative microchip capillary electrophoresis

T. Revermann

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    22 Downloads (Pure)

    Abstract

    The development of novel instrumentation and analytical methodology for quantitative microchip capillary electrophoresis (MCE) is described in this thesis. Demanding only small quantities of reagents and samples, microfluidic instrumentation is highly advantageous. Fast separations at high voltages have been performed, which require the use of flexible high-voltage power supplies that have been developed in this work. An introductory overview of the general requirements of MCE is provided. Differences of chip-based CE and conventional CE are addressed, and the effects of buffer decomposition, reactions at electrodes, clogging of microchips, surface interactions, injection and power supply as well as detector-related problems on measurements are discussed. Additionally also strategies for proper analysis and technical solutions are identified.
    Original languageUndefined
    Awarding Institution
    • University of Twente
    Supervisors/Advisors
    • Karst, U., Supervisor
    Award date2 Feb 2007
    Place of PublicationEnschede
    Publisher
    Print ISBNs9789036524575
    Publication statusPublished - 2 Feb 2007

    Keywords

    • IR-58983

    Cite this

    Revermann, T. (2007). Methods and instrumentation for quantitative microchip capillary electrophoresis. Enschede: University of Twente.