Methods of monitoring cell fate and tissue growth in three-dimensional scaffold-based strategies for in vitro tissue engineering

Anne Marijke Leferink, Clemens van Blitterswijk, Lorenzo Moroni

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
162 Downloads (Pure)

Abstract

In the field of tissue engineering, there is a need for methods that allow assessing the performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date, histological analysis is the golden standard to retrieve information on tissue growth, cellular distribution, and cell fate on tissue-engineered constructs after in vitro cell culture or on explanted specimens after in vivo applications. Yet, many advances have been made to optimize imaging techniques for monitoring tissue-engineered constructs with a sub-mm or μm resolution. Many imaging modalities have first been developed for clinical applications, in which a high penetration depth has been often more important than lateral resolution. In this study, we have reviewed the current state of the art in several imaging approaches that have shown to be promising in monitoring cell fate and tissue growth upon in vitro culture. Depending on the aimed tissue type and scaffold properties, some imaging methods are more applicable than others. Optical methods are mostly suited for transparent materials such as hydrogels, whereas magnetic resonance-based methods are mostly applied to obtain contrast between hard and soft tissues regardless of their transparency. Overall, this review shows that the field of imaging in scaffold-based tissue engineering is developing at a fast pace and has the potential to overcome the limitations of destructive endpoint analysis.
Original languageUndefined
Pages (from-to)265-283
Number of pages19
JournalTissue engineering. Part B: Reviews
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Mar 2016

Keywords

  • EWI-27687
  • IR-103349
  • METIS-321699

Cite this

@article{7d903c3a1999491ba57d09eda2e63a0d,
title = "Methods of monitoring cell fate and tissue growth in three-dimensional scaffold-based strategies for in vitro tissue engineering",
abstract = "In the field of tissue engineering, there is a need for methods that allow assessing the performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date, histological analysis is the golden standard to retrieve information on tissue growth, cellular distribution, and cell fate on tissue-engineered constructs after in vitro cell culture or on explanted specimens after in vivo applications. Yet, many advances have been made to optimize imaging techniques for monitoring tissue-engineered constructs with a sub-mm or μm resolution. Many imaging modalities have first been developed for clinical applications, in which a high penetration depth has been often more important than lateral resolution. In this study, we have reviewed the current state of the art in several imaging approaches that have shown to be promising in monitoring cell fate and tissue growth upon in vitro culture. Depending on the aimed tissue type and scaffold properties, some imaging methods are more applicable than others. Optical methods are mostly suited for transparent materials such as hydrogels, whereas magnetic resonance-based methods are mostly applied to obtain contrast between hard and soft tissues regardless of their transparency. Overall, this review shows that the field of imaging in scaffold-based tissue engineering is developing at a fast pace and has the potential to overcome the limitations of destructive endpoint analysis.",
keywords = "EWI-27687, IR-103349, METIS-321699",
author = "Leferink, {Anne Marijke} and {van Blitterswijk}, Clemens and Lorenzo Moroni",
note = "10.1089/ten.TEB.2015.0340",
year = "2016",
month = "3",
day = "1",
doi = "10.1089/ten.TEB.2015.0340",
language = "Undefined",
volume = "22",
pages = "265--283",
journal = "Tissue engineering. Part B: Reviews",
issn = "1937-3368",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

Methods of monitoring cell fate and tissue growth in three-dimensional scaffold-based strategies for in vitro tissue engineering. / Leferink, Anne Marijke; van Blitterswijk, Clemens; Moroni, Lorenzo.

In: Tissue engineering. Part B: Reviews, Vol. 22, No. 4, 01.03.2016, p. 265-283.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Methods of monitoring cell fate and tissue growth in three-dimensional scaffold-based strategies for in vitro tissue engineering

AU - Leferink, Anne Marijke

AU - van Blitterswijk, Clemens

AU - Moroni, Lorenzo

N1 - 10.1089/ten.TEB.2015.0340

PY - 2016/3/1

Y1 - 2016/3/1

N2 - In the field of tissue engineering, there is a need for methods that allow assessing the performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date, histological analysis is the golden standard to retrieve information on tissue growth, cellular distribution, and cell fate on tissue-engineered constructs after in vitro cell culture or on explanted specimens after in vivo applications. Yet, many advances have been made to optimize imaging techniques for monitoring tissue-engineered constructs with a sub-mm or μm resolution. Many imaging modalities have first been developed for clinical applications, in which a high penetration depth has been often more important than lateral resolution. In this study, we have reviewed the current state of the art in several imaging approaches that have shown to be promising in monitoring cell fate and tissue growth upon in vitro culture. Depending on the aimed tissue type and scaffold properties, some imaging methods are more applicable than others. Optical methods are mostly suited for transparent materials such as hydrogels, whereas magnetic resonance-based methods are mostly applied to obtain contrast between hard and soft tissues regardless of their transparency. Overall, this review shows that the field of imaging in scaffold-based tissue engineering is developing at a fast pace and has the potential to overcome the limitations of destructive endpoint analysis.

AB - In the field of tissue engineering, there is a need for methods that allow assessing the performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date, histological analysis is the golden standard to retrieve information on tissue growth, cellular distribution, and cell fate on tissue-engineered constructs after in vitro cell culture or on explanted specimens after in vivo applications. Yet, many advances have been made to optimize imaging techniques for monitoring tissue-engineered constructs with a sub-mm or μm resolution. Many imaging modalities have first been developed for clinical applications, in which a high penetration depth has been often more important than lateral resolution. In this study, we have reviewed the current state of the art in several imaging approaches that have shown to be promising in monitoring cell fate and tissue growth upon in vitro culture. Depending on the aimed tissue type and scaffold properties, some imaging methods are more applicable than others. Optical methods are mostly suited for transparent materials such as hydrogels, whereas magnetic resonance-based methods are mostly applied to obtain contrast between hard and soft tissues regardless of their transparency. Overall, this review shows that the field of imaging in scaffold-based tissue engineering is developing at a fast pace and has the potential to overcome the limitations of destructive endpoint analysis.

KW - EWI-27687

KW - IR-103349

KW - METIS-321699

U2 - 10.1089/ten.TEB.2015.0340

DO - 10.1089/ten.TEB.2015.0340

M3 - Article

VL - 22

SP - 265

EP - 283

JO - Tissue engineering. Part B: Reviews

JF - Tissue engineering. Part B: Reviews

SN - 1937-3368

IS - 4

ER -