Micro- and Nanofabrication of Robust Reactive Arrays Based on the Covalent Coupling of Dendrimers to Activated Monolayers

Geerten H. Degenhart, B. Dordi, Holger Schönherr, Gyula J. Vancso

Research output: Contribution to journalArticleAcademic

54 Citations (Scopus)

Abstract

We report on methods to fabricate robust micro- and nanopatterned platforms, comprising high functional group densities and quasi three-dimensional structures, for possible applications in biochip array technologies. For this purpose, amine-terminated poly(amidoamine) (PAMAM) dendrimers were immobilized via amide linkage formation on 11,11'-dithiobis(N-hydroxysuccinimidylundecanoate) (NHS-C10) self-assembled monolayers (SAMs) on gold surfaces. The coupling reaction and the resulting assemblies were characterized by grazing incidence reflection Fourier transform infrared spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy; the obtained surface coverage values were successfully fitted with a Langmuir isotherm. The fraction of unreacted peripheral primary amine groups of the surface-immobilized PAMAM dendrimers was 28% as determined by XPS analysis of trifluoroacetic anhydride-labeled assemblies. Patterning of the PAMAM dendrimers on NHS-C10 SAMs on the micrometer and sub-100-nm scale was achieved by microcontact printing and dip pen nanolithography. The resulting patterns are characterized by their high degree of order and stability of the transferred molecules due to covalent attachment.
Original languageUndefined
Pages (from-to)6216-6224
JournalLangmuir
Volume20
Issue number15
DOIs
Publication statusPublished - 2004

Keywords

  • IR-59364

Cite this

Degenhart, Geerten H. ; Dordi, B. ; Schönherr, Holger ; Vancso, Gyula J. / Micro- and Nanofabrication of Robust Reactive Arrays Based on the Covalent Coupling of Dendrimers to Activated Monolayers. In: Langmuir. 2004 ; Vol. 20, No. 15. pp. 6216-6224.
@article{4a1abda636684633b2bc2fc77b4b2c8e,
title = "Micro- and Nanofabrication of Robust Reactive Arrays Based on the Covalent Coupling of Dendrimers to Activated Monolayers",
abstract = "We report on methods to fabricate robust micro- and nanopatterned platforms, comprising high functional group densities and quasi three-dimensional structures, for possible applications in biochip array technologies. For this purpose, amine-terminated poly(amidoamine) (PAMAM) dendrimers were immobilized via amide linkage formation on 11,11'-dithiobis(N-hydroxysuccinimidylundecanoate) (NHS-C10) self-assembled monolayers (SAMs) on gold surfaces. The coupling reaction and the resulting assemblies were characterized by grazing incidence reflection Fourier transform infrared spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy; the obtained surface coverage values were successfully fitted with a Langmuir isotherm. The fraction of unreacted peripheral primary amine groups of the surface-immobilized PAMAM dendrimers was 28{\%} as determined by XPS analysis of trifluoroacetic anhydride-labeled assemblies. Patterning of the PAMAM dendrimers on NHS-C10 SAMs on the micrometer and sub-100-nm scale was achieved by microcontact printing and dip pen nanolithography. The resulting patterns are characterized by their high degree of order and stability of the transferred molecules due to covalent attachment.",
keywords = "IR-59364",
author = "Degenhart, {Geerten H.} and B. Dordi and Holger Sch{\"o}nherr and Vancso, {Gyula J.}",
year = "2004",
doi = "10.1021/la049580u",
language = "Undefined",
volume = "20",
pages = "6216--6224",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "15",

}

Micro- and Nanofabrication of Robust Reactive Arrays Based on the Covalent Coupling of Dendrimers to Activated Monolayers. / Degenhart, Geerten H.; Dordi, B.; Schönherr, Holger; Vancso, Gyula J.

In: Langmuir, Vol. 20, No. 15, 2004, p. 6216-6224.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Micro- and Nanofabrication of Robust Reactive Arrays Based on the Covalent Coupling of Dendrimers to Activated Monolayers

AU - Degenhart, Geerten H.

AU - Dordi, B.

AU - Schönherr, Holger

AU - Vancso, Gyula J.

PY - 2004

Y1 - 2004

N2 - We report on methods to fabricate robust micro- and nanopatterned platforms, comprising high functional group densities and quasi three-dimensional structures, for possible applications in biochip array technologies. For this purpose, amine-terminated poly(amidoamine) (PAMAM) dendrimers were immobilized via amide linkage formation on 11,11'-dithiobis(N-hydroxysuccinimidylundecanoate) (NHS-C10) self-assembled monolayers (SAMs) on gold surfaces. The coupling reaction and the resulting assemblies were characterized by grazing incidence reflection Fourier transform infrared spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy; the obtained surface coverage values were successfully fitted with a Langmuir isotherm. The fraction of unreacted peripheral primary amine groups of the surface-immobilized PAMAM dendrimers was 28% as determined by XPS analysis of trifluoroacetic anhydride-labeled assemblies. Patterning of the PAMAM dendrimers on NHS-C10 SAMs on the micrometer and sub-100-nm scale was achieved by microcontact printing and dip pen nanolithography. The resulting patterns are characterized by their high degree of order and stability of the transferred molecules due to covalent attachment.

AB - We report on methods to fabricate robust micro- and nanopatterned platforms, comprising high functional group densities and quasi three-dimensional structures, for possible applications in biochip array technologies. For this purpose, amine-terminated poly(amidoamine) (PAMAM) dendrimers were immobilized via amide linkage formation on 11,11'-dithiobis(N-hydroxysuccinimidylundecanoate) (NHS-C10) self-assembled monolayers (SAMs) on gold surfaces. The coupling reaction and the resulting assemblies were characterized by grazing incidence reflection Fourier transform infrared spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy; the obtained surface coverage values were successfully fitted with a Langmuir isotherm. The fraction of unreacted peripheral primary amine groups of the surface-immobilized PAMAM dendrimers was 28% as determined by XPS analysis of trifluoroacetic anhydride-labeled assemblies. Patterning of the PAMAM dendrimers on NHS-C10 SAMs on the micrometer and sub-100-nm scale was achieved by microcontact printing and dip pen nanolithography. The resulting patterns are characterized by their high degree of order and stability of the transferred molecules due to covalent attachment.

KW - IR-59364

U2 - 10.1021/la049580u

DO - 10.1021/la049580u

M3 - Article

VL - 20

SP - 6216

EP - 6224

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 15

ER -