Micro cell vesicle technology (mCVT): A novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

Yi Hsuan Ou; Shui Zou; Wei Jiang Goh; Suet Yen Chong; Gopalakrishnan Venkatesan; Matthias G. Wacker; Gerrit Storm; Bertrand Czarny; Giorgia Pastorin; Esther C.Y. Woon

doi:10.1039/d0nr03784b

Micro cell vesicle technology (mCVT): A novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

Yi Hsuan Ou
, Shui Zou
, Wei Jiang Goh
, Suet Yen Chong
, Gopalakrishnan Venkatesan
, Matthias G. Wacker
, Gerrit Storm
, Bertrand Czarny
, Giorgia Pastorin
, Esther C.Y. Woon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

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Abstract

A hybrid gene delivery platform, micro Cell Vesicle Technology (mCVT), produced from the fusion of plasma membranes and cationic lipids, is presently used to improve the transfection efficiency of hard-to-transfect (HTT) cells. The plasma membrane components of mCVTs impart specificity in cellular uptake and reduce cytotoxicity in the transfection process, while the cationic lipids complex with the genetic material and provide structural integrity to mCVTs. This journal is

Original language	English
Pages (from-to)	18022-18030
Number of pages	9
Journal	Nanoscale
Volume	12
Issue number	35
DOIs	https://doi.org/10.1039/d0nr03784b
Publication status	Published - 21 Sept 2020

Keywords

22/2 OA procedure

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10.1039/d0nr03784b

Ou-2020-Micro-cell-vesicle-technology-mcvt-Final published version, 2.25 MBLicence: Taverne

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title = "Micro cell vesicle technology (mCVT): A novel hybrid system of gene delivery for hard-to-transfect (HTT) cells",

abstract = "A hybrid gene delivery platform, micro Cell Vesicle Technology (mCVT), produced from the fusion of plasma membranes and cationic lipids, is presently used to improve the transfection efficiency of hard-to-transfect (HTT) cells. The plasma membrane components of mCVTs impart specificity in cellular uptake and reduce cytotoxicity in the transfection process, while the cationic lipids complex with the genetic material and provide structural integrity to mCVTs. This journal is ",

keywords = "22/2 OA procedure",

author = "Ou, \{Yi Hsuan\} and Shui Zou and Goh, \{Wei Jiang\} and Chong, \{Suet Yen\} and Gopalakrishnan Venkatesan and Wacker, \{Matthias G.\} and Gerrit Storm and Bertrand Czarny and Giorgia Pastorin and Woon, \{Esther C.Y.\}",

note = "Funding Information: This work was supported by National University of Singapore (NUS), Department of Pharmacy and by the funding provided by MOE (grant number R-148-000-296-114), Nanyang Technological University, Singapore (grant number 001487-00001), and partly supported by the Singapore NMRC (NMRC/ OFYIRG/0081/2018 to J. W. W., G. S., B. C. and G. P.). The authors would like to acknowledge A/P Gigi Chiu and A/P Ang Wee Han in NUS for the use of cells and equipment. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

year = "2020",

month = sep,

day = "21",

doi = "10.1039/d0nr03784b",

language = "English",

volume = "12",

pages = "18022--18030",

journal = "Nanoscale",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

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T2 - A novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

AU - Ou, Yi Hsuan

AU - Zou, Shui

AU - Goh, Wei Jiang

AU - Chong, Suet Yen

AU - Venkatesan, Gopalakrishnan

AU - Wacker, Matthias G.

AU - Storm, Gerrit

AU - Czarny, Bertrand

AU - Pastorin, Giorgia

AU - Woon, Esther C.Y.

N1 - Funding Information: This work was supported by National University of Singapore (NUS), Department of Pharmacy and by the funding provided by MOE (grant number R-148-000-296-114), Nanyang Technological University, Singapore (grant number 001487-00001), and partly supported by the Singapore NMRC (NMRC/ OFYIRG/0081/2018 to J. W. W., G. S., B. C. and G. P.). The authors would like to acknowledge A/P Gigi Chiu and A/P Ang Wee Han in NUS for the use of cells and equipment. Publisher Copyright: © 2020 The Royal Society of Chemistry.

PY - 2020/9/21

Y1 - 2020/9/21

N2 - A hybrid gene delivery platform, micro Cell Vesicle Technology (mCVT), produced from the fusion of plasma membranes and cationic lipids, is presently used to improve the transfection efficiency of hard-to-transfect (HTT) cells. The plasma membrane components of mCVTs impart specificity in cellular uptake and reduce cytotoxicity in the transfection process, while the cationic lipids complex with the genetic material and provide structural integrity to mCVTs. This journal is

AB - A hybrid gene delivery platform, micro Cell Vesicle Technology (mCVT), produced from the fusion of plasma membranes and cationic lipids, is presently used to improve the transfection efficiency of hard-to-transfect (HTT) cells. The plasma membrane components of mCVTs impart specificity in cellular uptake and reduce cytotoxicity in the transfection process, while the cationic lipids complex with the genetic material and provide structural integrity to mCVTs. This journal is

KW - 22/2 OA procedure

UR - https://www.scopus.com/pages/publications/85091263571

U2 - 10.1039/d0nr03784b

DO - 10.1039/d0nr03784b

M3 - Article

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AN - SCOPUS:85091263571

SN - 2040-3364

VL - 12

SP - 18022

EP - 18030

JO - Nanoscale

JF - Nanoscale

IS - 35

ER -

Micro cell vesicle technology (mCVT): A novel hybrid system of gene delivery for hard-to-transfect (HTT) cells

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