Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line

Bach Q. Le, Aliaksei Vasilevich, Steven Vermeulen, Frits Hulshof, Dimitrios F. Stamatialis, Clemens A. van Blitterswijk, Jan de Boer

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Chemical and mechanical cues are well-established influencers of in vitro chondrogenic differentiation of ATDC5 cells. Here, we investigate the role of topographical cues in this differentiation process, a study not been explored before. Previously, using a library of surface micro-topographies we found some distinct patterns that induced alkaline phosphatase (ALP) production in human mesenchymal stromal cells. ALP is also a marker for hypertrophy, the end stage of chondrogenic differentiation preceding bone formation. Thus, we hypothesized that these patterns could influence end-stage chondrogenic differentiation of ATDC5 cells. In this study, we randomly selected seven topographies among the ALP influencing hits. Cells grown on these surfaces displayed varying nuclear shape and actin filament structure. When stimulated with insulin-transferrin-selenium (ITS) medium, nodule formation occurred and in some cases showed alignment to the topographical patterns. Gene expression analysis of cells growing on topographical surfaces in the presence of ITS medium revealed a downregulation of early markers and upregulation of late markers of chondrogenic differentiation compared to cells grown on a flat surface. In conclusion, we demonstrated that surface topography in addition to other cues can promote hypertrophic differentiation suitable for bone tissue engineering.

Original languageEnglish
Pages (from-to)458-469
Number of pages12
JournalTissue engineering. Part A
Volume23
Issue number9-10
DOIs
Publication statusPublished - 1 May 2017

Fingerprint

Differentiation Antigens
Topography
Cues
Alkaline Phosphatase
Phosphatases
Cells
Transferrin
Selenium
Cell Line
Cell Differentiation
Insulin
Bone
Tissue Engineering
Mesenchymal Stromal Cells
Actin Cytoskeleton
Osteogenesis
Hypertrophy
Libraries
Surface topography
Up-Regulation

Keywords

  • ATDC5
  • cell morphology
  • chondrogenesis
  • hypertrophy
  • Surface topography

Cite this

Le, B. Q., Vasilevich, A., Vermeulen, S., Hulshof, F., Stamatialis, D. F., van Blitterswijk, C. A., & de Boer, J. (2017). Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line. Tissue engineering. Part A, 23(9-10), 458-469. https://doi.org/10.1089/ten.tea.2016.0421
Le, Bach Q. ; Vasilevich, Aliaksei ; Vermeulen, Steven ; Hulshof, Frits ; Stamatialis, Dimitrios F. ; van Blitterswijk, Clemens A. ; de Boer, Jan. / Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line. In: Tissue engineering. Part A. 2017 ; Vol. 23, No. 9-10. pp. 458-469.
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Le, BQ, Vasilevich, A, Vermeulen, S, Hulshof, F, Stamatialis, DF, van Blitterswijk, CA & de Boer, J 2017, 'Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line' Tissue engineering. Part A, vol. 23, no. 9-10, pp. 458-469. https://doi.org/10.1089/ten.tea.2016.0421

Micro-Topographies Promote Late Chondrogenic Differentiation Markers in the ATDC5 Cell Line. / Le, Bach Q.; Vasilevich, Aliaksei; Vermeulen, Steven; Hulshof, Frits; Stamatialis, Dimitrios F.; van Blitterswijk, Clemens A.; de Boer, Jan.

In: Tissue engineering. Part A, Vol. 23, No. 9-10, 01.05.2017, p. 458-469.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Stamatialis, Dimitrios F.

AU - van Blitterswijk, Clemens A.

AU - de Boer, Jan

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AB - Chemical and mechanical cues are well-established influencers of in vitro chondrogenic differentiation of ATDC5 cells. Here, we investigate the role of topographical cues in this differentiation process, a study not been explored before. Previously, using a library of surface micro-topographies we found some distinct patterns that induced alkaline phosphatase (ALP) production in human mesenchymal stromal cells. ALP is also a marker for hypertrophy, the end stage of chondrogenic differentiation preceding bone formation. Thus, we hypothesized that these patterns could influence end-stage chondrogenic differentiation of ATDC5 cells. In this study, we randomly selected seven topographies among the ALP influencing hits. Cells grown on these surfaces displayed varying nuclear shape and actin filament structure. When stimulated with insulin-transferrin-selenium (ITS) medium, nodule formation occurred and in some cases showed alignment to the topographical patterns. Gene expression analysis of cells growing on topographical surfaces in the presence of ITS medium revealed a downregulation of early markers and upregulation of late markers of chondrogenic differentiation compared to cells grown on a flat surface. In conclusion, we demonstrated that surface topography in addition to other cues can promote hypertrophic differentiation suitable for bone tissue engineering.

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