Microbubbles reforming endothelium

Annemieke Van Wamel; Klazina Kooiman; Miranda Harteveld; Marcia Emmer; Folkert J. Ten Cate; Michel Versluis; Nico De Jong

doi:10.1063/1.2205431

Microbubbles reforming endothelium

Annemieke Van Wamel^*
, Klazina Kooiman
, Miranda Harteveld
, Marcia Emmer
, Folkert J. Ten Cate
, Michel Versluis
, Nico De Jong

^*Corresponding author for this work

Physics of Fluids

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Ultrasound contrast microbubbles have the ability to enhance cell permeability and thus may be used as a new way to deliver drugs inter-vascular. It facilitates the transfer of extracellular molecules into cells through ultrasound-activated microbubbles. The present study is designed to correlate the relationship between microbubble induced cell deformation and enhanced cell membrane permeability. Propidium Iodide was used as a membrane integrity probe. Using high-speed imaging of vibrating microbubbles against endothelial cells and imaging transport of Propidium Iodide into these cells showed a direct correlation between cell deformation and resulting cell membrane permeability. The membrane permeabilization lasted for a short period without affecting aortic endothelial cells viability. We identified that microbubbles are crucial to enhance transient cell membrane permeability.

Original language	English
Title of host publication	THERAPEUTIC ULTRASOUND
Subtitle of host publication	5th International Symposium on Therapeutic Ultrasound
Pages	24-28
Number of pages	5
DOIs	https://doi.org/10.1063/1.2205431
Publication status	Published - 8 May 2006
Event	5th International Symposium on Therapeutic Ultrasound, ISTU 2005 - Boston, United States Duration: 27 Oct 2005 → 29 Oct 2005 Conference number: 5

Publication series

Name	AIP Conference Proceedings
Volume	829
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	5th International Symposium on Therapeutic Ultrasound, ISTU 2005
Abbreviated title	ISTU
Country/Territory	United States
City	Boston
Period	27/10/05 → 29/10/05

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10.1063/1.2205431

Cite this

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title = "Microbubbles reforming endothelium",

abstract = "Ultrasound contrast microbubbles have the ability to enhance cell permeability and thus may be used as a new way to deliver drugs inter-vascular. It facilitates the transfer of extracellular molecules into cells through ultrasound-activated microbubbles. The present study is designed to correlate the relationship between microbubble induced cell deformation and enhanced cell membrane permeability. Propidium Iodide was used as a membrane integrity probe. Using high-speed imaging of vibrating microbubbles against endothelial cells and imaging transport of Propidium Iodide into these cells showed a direct correlation between cell deformation and resulting cell membrane permeability. The membrane permeabilization lasted for a short period without affecting aortic endothelial cells viability. We identified that microbubbles are crucial to enhance transient cell membrane permeability.",

author = "\{Van Wamel\}, Annemieke and Klazina Kooiman and Miranda Harteveld and Marcia Emmer and \{Ten Cate\}, \{Folkert J.\} and Michel Versluis and \{De Jong\}, Nico",

year = "2006",

month = may,

day = "8",

doi = "10.1063/1.2205431",

language = "English",

isbn = "073540321X",

series = "AIP Conference Proceedings",

pages = "24--28",

booktitle = "THERAPEUTIC ULTRASOUND",

note = "5th International Symposium on Therapeutic Ultrasound, ISTU 2005, ISTU ; Conference date: 27-10-2005 Through 29-10-2005",

}

Van Wamel, A, Kooiman, K, Harteveld, M, Emmer, M, Ten Cate, FJ, Versluis, M & De Jong, N 2006, Microbubbles reforming endothelium. in THERAPEUTIC ULTRASOUND: 5th International Symposium on Therapeutic Ultrasound. AIP Conference Proceedings, vol. 829, pp. 24-28, 5th International Symposium on Therapeutic Ultrasound, ISTU 2005, Boston, Massachusetts, United States, 27/10/05. https://doi.org/10.1063/1.2205431

TY - GEN

T1 - Microbubbles reforming endothelium

AU - Van Wamel, Annemieke

AU - Kooiman, Klazina

AU - Harteveld, Miranda

AU - Emmer, Marcia

AU - Ten Cate, Folkert J.

AU - Versluis, Michel

AU - De Jong, Nico

N1 - Conference code: 5

PY - 2006/5/8

Y1 - 2006/5/8

N2 - Ultrasound contrast microbubbles have the ability to enhance cell permeability and thus may be used as a new way to deliver drugs inter-vascular. It facilitates the transfer of extracellular molecules into cells through ultrasound-activated microbubbles. The present study is designed to correlate the relationship between microbubble induced cell deformation and enhanced cell membrane permeability. Propidium Iodide was used as a membrane integrity probe. Using high-speed imaging of vibrating microbubbles against endothelial cells and imaging transport of Propidium Iodide into these cells showed a direct correlation between cell deformation and resulting cell membrane permeability. The membrane permeabilization lasted for a short period without affecting aortic endothelial cells viability. We identified that microbubbles are crucial to enhance transient cell membrane permeability.

AB - Ultrasound contrast microbubbles have the ability to enhance cell permeability and thus may be used as a new way to deliver drugs inter-vascular. It facilitates the transfer of extracellular molecules into cells through ultrasound-activated microbubbles. The present study is designed to correlate the relationship between microbubble induced cell deformation and enhanced cell membrane permeability. Propidium Iodide was used as a membrane integrity probe. Using high-speed imaging of vibrating microbubbles against endothelial cells and imaging transport of Propidium Iodide into these cells showed a direct correlation between cell deformation and resulting cell membrane permeability. The membrane permeabilization lasted for a short period without affecting aortic endothelial cells viability. We identified that microbubbles are crucial to enhance transient cell membrane permeability.

UR - https://www.scopus.com/pages/publications/33845445623

U2 - 10.1063/1.2205431

DO - 10.1063/1.2205431

M3 - Conference contribution

AN - SCOPUS:33845445623

SN - 073540321X

SN - 9780735403215

T3 - AIP Conference Proceedings

SP - 24

EP - 28

BT - THERAPEUTIC ULTRASOUND

T2 - 5th International Symposium on Therapeutic Ultrasound, ISTU 2005

Y2 - 27 October 2005 through 29 October 2005

ER -

Microbubbles reforming endothelium

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