Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass

Dean De Boer; Torben W. van Voorst; Erwin J.W. Berenschot; L. Niels Cornelisse; Niels R. Tas

Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass

Dean De Boer
, Torben W. van Voorst
, Erwin J.W. Berenschot
, L. Niels Cornelisse^*
, Niels R. Tas^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

73 Downloads (Pure)

Abstract

We present a microfluidic device for creating defined neural microcircuits by directional axon guidance of two distinct neuron populations. Directional guiding is achieved by a modified version of the 'stomach' shape developed by Forró [1], and two different cell population are made possible by a multilayer silicon-glass construction. Preliminary experiments were conducted by culturing neurons for 2 weeks, with fluorescent microscopy suggesting directional network formation based on two distinct neuron populations. This opens a wide range of possibilities in creating a platform for biological models of more advanced neural microcircuits consisting of distinct cell lines and neuronal subtypes.

Original language	English
Title of host publication	2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
Place of Publication	Piscataway, NJ
Publisher	IEEE
Pages	2169-2172
Number of pages	4
ISBN (Electronic)	978-4-88686-435-2
ISBN (Print)	979-8-3503-3302-2
Publication status	Published - 7 May 2024
Event	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 - Kyoto, Japan Duration: 25 Jun 2023 → 29 Jun 2023 Conference number: 22

Publication series

Name	Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)
Publisher	IEEE
Number	22
Volume	2023
ISSN (Print)	2167-0013
ISSN (Electronic)	2167-0021

Conference

Conference	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
Abbreviated title	Transducers 2023
Country/Territory	Japan
City	Kyoto
Period	25/06/23 → 29/06/23

Keywords

2024 OA procedure
Convergent
Directional
Glass
Microcircuit
Microfluidic
Network
Neuron
Silicon
SOI
Axon guiding

Access to Document

ArticleFinal published version, 1.7 MBLicence: Taverne

https://ieeexplore.ieee.org/document/10516703

Cite this

De Boer, D., van Voorst, T. W., Berenschot, E. J. W., Cornelisse, L. N., & Tas, N. R. (2024). Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass. In 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 (pp. 2169-2172). (Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers); Vol. 2023, No. 22). IEEE. https://ieeexplore.ieee.org/document/10516703

De Boer, Dean ; van Voorst, Torben W. ; Berenschot, Erwin J.W. et al. / Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass. 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Piscataway, NJ : IEEE, 2024. pp. 2169-2172 (Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers); 22).

@inproceedings{4bfbbf2a75ce4dbe988f316fd5403a8c,

title = "Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass",

abstract = "We present a microfluidic device for creating defined neural microcircuits by directional axon guidance of two distinct neuron populations. Directional guiding is achieved by a modified version of the 'stomach' shape developed by Forr{\'o} [1], and two different cell population are made possible by a multilayer silicon-glass construction. Preliminary experiments were conducted by culturing neurons for 2 weeks, with fluorescent microscopy suggesting directional network formation based on two distinct neuron populations. This opens a wide range of possibilities in creating a platform for biological models of more advanced neural microcircuits consisting of distinct cell lines and neuronal subtypes.",

keywords = "2024 OA procedure, Convergent, Directional, Glass, Microcircuit, Microfluidic, Network, Neuron, Silicon, SOI, Axon guiding",

author = "\{De Boer\}, Dean and \{van Voorst\}, \{Torben W.\} and Berenschot, \{Erwin J.W.\} and Cornelisse, \{L. Niels\} and Tas, \{Niels R.\}",

note = "Publisher Copyright: {\textcopyright} 2023 IEEJ.; 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Transducers 2023 ; Conference date: 25-06-2023 Through 29-06-2023",

year = "2024",

month = may,

day = "7",

language = "English",

isbn = "979-8-3503-3302-2",

series = "Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)",

publisher = "IEEE",

number = "22",

pages = "2169--2172",

booktitle = "2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023",

address = "United States",

}

De Boer, D, van Voorst, TW, Berenschot, EJW, Cornelisse, LN & Tas, NR 2024, Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass. in 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers), no. 22, vol. 2023, IEEE, Piscataway, NJ, pp. 2169-2172, 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Kyoto, Japan, 25/06/23. <https://ieeexplore.ieee.org/document/10516703>

Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass. / De Boer, Dean; van Voorst, Torben W.; Berenschot, Erwin J.W. et al.
2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Piscataway, NJ: IEEE, 2024. p. 2169-2172 (Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers); Vol. 2023, No. 22).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass

AU - De Boer, Dean

AU - van Voorst, Torben W.

AU - Berenschot, Erwin J.W.

AU - Cornelisse, L. Niels

AU - Tas, Niels R.

N1 - Conference code: 22

PY - 2024/5/7

Y1 - 2024/5/7

N2 - We present a microfluidic device for creating defined neural microcircuits by directional axon guidance of two distinct neuron populations. Directional guiding is achieved by a modified version of the 'stomach' shape developed by Forró [1], and two different cell population are made possible by a multilayer silicon-glass construction. Preliminary experiments were conducted by culturing neurons for 2 weeks, with fluorescent microscopy suggesting directional network formation based on two distinct neuron populations. This opens a wide range of possibilities in creating a platform for biological models of more advanced neural microcircuits consisting of distinct cell lines and neuronal subtypes.

AB - We present a microfluidic device for creating defined neural microcircuits by directional axon guidance of two distinct neuron populations. Directional guiding is achieved by a modified version of the 'stomach' shape developed by Forró [1], and two different cell population are made possible by a multilayer silicon-glass construction. Preliminary experiments were conducted by culturing neurons for 2 weeks, with fluorescent microscopy suggesting directional network formation based on two distinct neuron populations. This opens a wide range of possibilities in creating a platform for biological models of more advanced neural microcircuits consisting of distinct cell lines and neuronal subtypes.

KW - 2024 OA procedure

KW - Convergent

KW - Directional

KW - Glass

KW - Microcircuit

KW - Microfluidic

KW - Network

KW - Neuron

KW - Silicon

KW - SOI

KW - Axon guiding

M3 - Conference contribution

AN - SCOPUS:85193468858

SN - 979-8-3503-3302-2

T3 - Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)

SP - 2169

EP - 2172

BT - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

PB - IEEE

CY - Piscataway, NJ

T2 - 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

Y2 - 25 June 2023 through 29 June 2023

ER -

De Boer D, van Voorst TW, Berenschot EJW, Cornelisse LN, Tas NR. Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass. In 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Piscataway, NJ: IEEE. 2024. p. 2169-2172. (Proceedings International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers); 22).

Microfabricated Platform for Directional Neural Microcircuits in Silicon-Glass

Abstract

Publication series

Conference

Keywords

Access to Document

Fingerprint

Cite this