Microfabricated solid-state ion-selective electrode probe for measuring potassium in the living rodent brain: Compatibility with DC-EEG recordings to study spreading depression

Mathieu Odijk, E.J. van der Wouden, Wouter Olthuis, M.D. Ferrari, E.A. Tolner, A.M.J.M. van den Maagdenberg, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    13 Citations (Scopus)

    Abstract

    There is considerable need in neuroscience research to reliably measure concentrations of extracellular ions in the living brain as the dynamics of ion levels increasingly is considered to play a key role in the pathophysiology of many brain disorders. Unfortunately, most probes currently used for direct measurement of extracellular ion concentrations in living brain tissue in experimental animals have considerable limitations. They are custom-made glass capillary ion-selective microelectrodes that are fragile, timeconsuming to prepare, and can practically only be used to measure at one depth and one location in the brain. We here present the design and fabrication of a novel type of miniaturized probe that can simultaneously measure, in the brain of a living animal, multiple parameters relevant to the neurological phenomenon of spreading depression (SD) at multiple depths and locations. SD is characterized by a slowly propagating wave of initial neuronal and glial cell depolarization that is followed by depression of activity. SD is accompanied by a massive redistribution of ions, including K+, between intracellular and extracellular compartments and is considered important in brain diseases such as stroke, traumatic brain injury, and migraine with aura. This work focuses on the functionalization of fabricated probes for measuring changes in extracellular K+ concentration (and its associated changes in neuronal activity) using an ion-selective potentiometric sensor. We show that the choice of conductive polymer and the method of electro-deposition is critical to obtain low cross-sensitivity for pH and O-2. The sensor's response to IC is approximately linear between 2 and 40 mM with a voltage-response of 39 mV/log[K+]. Proof-of-principle in vivo brain recordings were performed in the cortex of a wild-type mouse during induction of cortical SD. We reliably measured the theoretically expected rise and fall of brain tissue K+ levels during a locally induced cortical SD. We envisage that our probe can be of great use to reliably measure K+, without being affected by pH or O-2 changes, in the living brain and be widely applicable in neuroscience research. (C) 2014 Elsevier B.V All rights reserved
    Original languageUndefined
    Pages (from-to)945-953
    Number of pages9
    JournalSensors and actuators. B: Chemical
    Volume207
    DOIs
    Publication statusPublished - Feb 2015

    Keywords

    • EWI-25617
    • METIS-309846
    • IR-93842

    Cite this

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    title = "Microfabricated solid-state ion-selective electrode probe for measuring potassium in the living rodent brain: Compatibility with DC-EEG recordings to study spreading depression",
    abstract = "There is considerable need in neuroscience research to reliably measure concentrations of extracellular ions in the living brain as the dynamics of ion levels increasingly is considered to play a key role in the pathophysiology of many brain disorders. Unfortunately, most probes currently used for direct measurement of extracellular ion concentrations in living brain tissue in experimental animals have considerable limitations. They are custom-made glass capillary ion-selective microelectrodes that are fragile, timeconsuming to prepare, and can practically only be used to measure at one depth and one location in the brain. We here present the design and fabrication of a novel type of miniaturized probe that can simultaneously measure, in the brain of a living animal, multiple parameters relevant to the neurological phenomenon of spreading depression (SD) at multiple depths and locations. SD is characterized by a slowly propagating wave of initial neuronal and glial cell depolarization that is followed by depression of activity. SD is accompanied by a massive redistribution of ions, including K+, between intracellular and extracellular compartments and is considered important in brain diseases such as stroke, traumatic brain injury, and migraine with aura. This work focuses on the functionalization of fabricated probes for measuring changes in extracellular K+ concentration (and its associated changes in neuronal activity) using an ion-selective potentiometric sensor. We show that the choice of conductive polymer and the method of electro-deposition is critical to obtain low cross-sensitivity for pH and O-2. The sensor's response to IC is approximately linear between 2 and 40 mM with a voltage-response of 39 mV/log[K+]. Proof-of-principle in vivo brain recordings were performed in the cortex of a wild-type mouse during induction of cortical SD. We reliably measured the theoretically expected rise and fall of brain tissue K+ levels during a locally induced cortical SD. We envisage that our probe can be of great use to reliably measure K+, without being affected by pH or O-2 changes, in the living brain and be widely applicable in neuroscience research. (C) 2014 Elsevier B.V All rights reserved",
    keywords = "EWI-25617, METIS-309846, IR-93842",
    author = "Mathieu Odijk and {van der Wouden}, E.J. and Wouter Olthuis and M.D. Ferrari and E.A. Tolner and {van den Maagdenberg}, A.M.J.M. and {van den Berg}, Albert",
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    Microfabricated solid-state ion-selective electrode probe for measuring potassium in the living rodent brain: Compatibility with DC-EEG recordings to study spreading depression. / Odijk, Mathieu; van der Wouden, E.J.; Olthuis, Wouter; Ferrari, M.D.; Tolner, E.A.; van den Maagdenberg, A.M.J.M.; van den Berg, Albert.

    In: Sensors and actuators. B: Chemical, Vol. 207, 02.2015, p. 945-953.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Microfabricated solid-state ion-selective electrode probe for measuring potassium in the living rodent brain: Compatibility with DC-EEG recordings to study spreading depression

    AU - Odijk, Mathieu

    AU - van der Wouden, E.J.

    AU - Olthuis, Wouter

    AU - Ferrari, M.D.

    AU - Tolner, E.A.

    AU - van den Maagdenberg, A.M.J.M.

    AU - van den Berg, Albert

    N1 - eemcs-eprint-25617

    PY - 2015/2

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    N2 - There is considerable need in neuroscience research to reliably measure concentrations of extracellular ions in the living brain as the dynamics of ion levels increasingly is considered to play a key role in the pathophysiology of many brain disorders. Unfortunately, most probes currently used for direct measurement of extracellular ion concentrations in living brain tissue in experimental animals have considerable limitations. They are custom-made glass capillary ion-selective microelectrodes that are fragile, timeconsuming to prepare, and can practically only be used to measure at one depth and one location in the brain. We here present the design and fabrication of a novel type of miniaturized probe that can simultaneously measure, in the brain of a living animal, multiple parameters relevant to the neurological phenomenon of spreading depression (SD) at multiple depths and locations. SD is characterized by a slowly propagating wave of initial neuronal and glial cell depolarization that is followed by depression of activity. SD is accompanied by a massive redistribution of ions, including K+, between intracellular and extracellular compartments and is considered important in brain diseases such as stroke, traumatic brain injury, and migraine with aura. This work focuses on the functionalization of fabricated probes for measuring changes in extracellular K+ concentration (and its associated changes in neuronal activity) using an ion-selective potentiometric sensor. We show that the choice of conductive polymer and the method of electro-deposition is critical to obtain low cross-sensitivity for pH and O-2. The sensor's response to IC is approximately linear between 2 and 40 mM with a voltage-response of 39 mV/log[K+]. Proof-of-principle in vivo brain recordings were performed in the cortex of a wild-type mouse during induction of cortical SD. We reliably measured the theoretically expected rise and fall of brain tissue K+ levels during a locally induced cortical SD. We envisage that our probe can be of great use to reliably measure K+, without being affected by pH or O-2 changes, in the living brain and be widely applicable in neuroscience research. (C) 2014 Elsevier B.V All rights reserved

    AB - There is considerable need in neuroscience research to reliably measure concentrations of extracellular ions in the living brain as the dynamics of ion levels increasingly is considered to play a key role in the pathophysiology of many brain disorders. Unfortunately, most probes currently used for direct measurement of extracellular ion concentrations in living brain tissue in experimental animals have considerable limitations. They are custom-made glass capillary ion-selective microelectrodes that are fragile, timeconsuming to prepare, and can practically only be used to measure at one depth and one location in the brain. We here present the design and fabrication of a novel type of miniaturized probe that can simultaneously measure, in the brain of a living animal, multiple parameters relevant to the neurological phenomenon of spreading depression (SD) at multiple depths and locations. SD is characterized by a slowly propagating wave of initial neuronal and glial cell depolarization that is followed by depression of activity. SD is accompanied by a massive redistribution of ions, including K+, between intracellular and extracellular compartments and is considered important in brain diseases such as stroke, traumatic brain injury, and migraine with aura. This work focuses on the functionalization of fabricated probes for measuring changes in extracellular K+ concentration (and its associated changes in neuronal activity) using an ion-selective potentiometric sensor. We show that the choice of conductive polymer and the method of electro-deposition is critical to obtain low cross-sensitivity for pH and O-2. The sensor's response to IC is approximately linear between 2 and 40 mM with a voltage-response of 39 mV/log[K+]. Proof-of-principle in vivo brain recordings were performed in the cortex of a wild-type mouse during induction of cortical SD. We reliably measured the theoretically expected rise and fall of brain tissue K+ levels during a locally induced cortical SD. We envisage that our probe can be of great use to reliably measure K+, without being affected by pH or O-2 changes, in the living brain and be widely applicable in neuroscience research. (C) 2014 Elsevier B.V All rights reserved

    KW - EWI-25617

    KW - METIS-309846

    KW - IR-93842

    U2 - 10.1016/j.snb.2014.06.138

    DO - 10.1016/j.snb.2014.06.138

    M3 - Article

    VL - 207

    SP - 945

    EP - 953

    JO - Sensors and actuators. B: Chemical

    JF - Sensors and actuators. B: Chemical

    SN - 0925-4005

    ER -