Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips

W.F. Quirós-Solano (Corresponding Author), N. Gaio, O.M.J.A. Stassen, Y.B. Arik, C. Silvestri, N.C.A. Van Engeland, A. van der Meer, R. Passier, C.M. Sahlgren, C.V.C. Bouten, A. van den Berg, R. Dekker, P.M. Sarro

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Abstract

We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65%) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85%). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.

Original languageEnglish
Article number13524
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 10 Sep 2018

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Membranes
Cells
Fabrication
Endothelial cells
Physiology
Pathology
Silicon
Biocompatibility
Molding
Pore size
MEMS
Scalability
Monolayers
Porosity

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Quirós-Solano, W. F., Gaio, N., Stassen, O. M. J. A., Arik, Y. B., Silvestri, C., Van Engeland, N. C. A., ... Sarro, P. M. (2018). Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. Scientific reports, 8(1), [13524]. https://doi.org/10.1038/s41598-018-31912-6
Quirós-Solano, W.F. ; Gaio, N. ; Stassen, O.M.J.A. ; Arik, Y.B. ; Silvestri, C. ; Van Engeland, N.C.A. ; van der Meer, A. ; Passier, R. ; Sahlgren, C.M. ; Bouten, C.V.C. ; van den Berg, A. ; Dekker, R. ; Sarro, P.M. / Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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abstract = "We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65{\%}) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85{\%}). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.",
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Quirós-Solano, WF, Gaio, N, Stassen, OMJA, Arik, YB, Silvestri, C, Van Engeland, NCA, van der Meer, A, Passier, R, Sahlgren, CM, Bouten, CVC, van den Berg, A, Dekker, R & Sarro, PM 2018, 'Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips' Scientific reports, vol. 8, no. 1, 13524. https://doi.org/10.1038/s41598-018-31912-6

Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. / Quirós-Solano, W.F. (Corresponding Author); Gaio, N.; Stassen, O.M.J.A.; Arik, Y.B.; Silvestri, C.; Van Engeland, N.C.A.; van der Meer, A.; Passier, R.; Sahlgren, C.M.; Bouten, C.V.C.; van den Berg, A.; Dekker, R.; Sarro, P.M.

In: Scientific reports, Vol. 8, No. 1, 13524, 10.09.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips

AU - Quirós-Solano, W.F.

AU - Gaio, N.

AU - Stassen, O.M.J.A.

AU - Arik, Y.B.

AU - Silvestri, C.

AU - Van Engeland, N.C.A.

AU - van der Meer, A.

AU - Passier, R.

AU - Sahlgren, C.M.

AU - Bouten, C.V.C.

AU - van den Berg, A.

AU - Dekker, R.

AU - Sarro, P.M.

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Y1 - 2018/9/10

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AB - We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65%) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85%). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.

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Quirós-Solano WF, Gaio N, Stassen OMJA, Arik YB, Silvestri C, Van Engeland NCA et al. Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. Scientific reports. 2018 Sep 10;8(1). 13524. https://doi.org/10.1038/s41598-018-31912-6