Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy

F. Wolbers; H.R. Franke; Helene Andersson; I. Vermes; I. Vermes; Albert van den Berg

Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy

F. Wolbers, H.R. Franke, Helene Andersson, I. Vermes, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Currently, the knowledge on breast cancer treatment is increasing, however, there are still hardly any assays present to match patients to the right form of therapy to enhance the therapeutic effectiveness. Therefore a microfluidic device is developed to analyze the hormonal and chemosensitivity of first breast cancer cell lines (MCF-7) and in a later stage a patients’ own tumor cells. The apoptotic inducers tumor ne-crosis factor-α, in combination with cycloheximide, and staurosporine stained MCF-7 cells positive for Annexin V and PI, and showed a decrease in area coverage as compared to untreated cells.

Original language	Undefined
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	L.E. Locascio, M. Gaitan, B.M. Paegel, D.J. Ross, W.N. Vreeland
Place of Publication	San Diego
Publisher	The Chemical and Biological Microsystems Society
Pages	549-551
Number of pages	3
ISBN (Print)	978-0-9798064-1-4
Publication status	Published - 12 Oct 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008 - San Diego, United States Duration: 12 Oct 2008 → 16 Oct 2008 Conference number: 12

Publication series

Name	Micro Total Analysis Systems
Publisher	Chemical and Biological Micro Systems Society
Volume	1
ISSN (Print)	1556-5890

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008
Abbreviated title	MicroTAS
Country/Territory	United States
City	San Diego
Period	12/10/08 → 16/10/08

Keywords

EWI-14978
METIS-256143
IR-62715

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

http://eprints.eemcs.utwente.nl/secure2/14978/01/uTas2008_0745_Wolbers_Manuscript.pdf

Cite this

Wolbers, F., Franke, H. R., Andersson, H., Vermes, I., Vermes, I., & van den Berg, A. (2008). Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy. In L. E. Locascio, M. Gaitan, B. M. Paegel, D. J. Ross, & W. N. Vreeland (Eds.), 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 549-551). (Micro Total Analysis Systems; Vol. 1). The Chemical and Biological Microsystems Society. http://eprints.eemcs.utwente.nl/secure2/14978/01/uTas2008_0745_Wolbers_Manuscript.pdf

Wolbers, F. ; Franke, H.R. ; Andersson, Helene et al. / Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / L.E. Locascio ; M. Gaitan ; B.M. Paegel ; D.J. Ross ; W.N. Vreeland. San Diego : The Chemical and Biological Microsystems Society, 2008. pp. 549-551 (Micro Total Analysis Systems).

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title = "Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy",

abstract = "Currently, the knowledge on breast cancer treatment is increasing, however, there are still hardly any assays present to match patients to the right form of therapy to enhance the therapeutic effectiveness. Therefore a microfluidic device is developed to analyze the hormonal and chemosensitivity of first breast cancer cell lines (MCF-7) and in a later stage a patients{\textquoteright} own tumor cells. The apoptotic inducers tumor ne-crosis factor-α, in combination with cycloheximide, and staurosporine stained MCF-7 cells positive for Annexin V and PI, and showed a decrease in area coverage as compared to untreated cells.",

keywords = "EWI-14978, METIS-256143, IR-62715",

author = "F. Wolbers and H.R. Franke and Helene Andersson and I. Vermes and I. Vermes and {van den Berg}, Albert",

note = "http://eprints.eemcs.utwente.nl/14978 ; 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

year = "2008",

month = oct,

day = "12",

language = "Undefined",

isbn = "978-0-9798064-1-4",

series = "Micro Total Analysis Systems",

publisher = "The Chemical and Biological Microsystems Society",

pages = "549--551",

editor = "L.E. Locascio and M. Gaitan and B.M. Paegel and D.J. Ross and W.N. Vreeland",

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Wolbers, F, Franke, HR, Andersson, H, Vermes, I, Vermes, I & van den Berg, A 2008, Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy. in LE Locascio, M Gaitan, BM Paegel, DJ Ross & WN Vreeland (eds), 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems, vol. 1, The Chemical and Biological Microsystems Society, San Diego, pp. 549-551, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008, San Diego, California, United States, 12/10/08. <http://eprints.eemcs.utwente.nl/secure2/14978/01/uTas2008_0745_Wolbers_Manuscript.pdf>

Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy. / Wolbers, F.; Franke, H.R.; Andersson, Helene et al.
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / L.E. Locascio; M. Gaitan; B.M. Paegel; D.J. Ross; W.N. Vreeland. San Diego: The Chemical and Biological Microsystems Society, 2008. p. 549-551 (Micro Total Analysis Systems; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy

AU - Wolbers, F.

AU - Franke, H.R.

AU - Andersson, Helene

AU - Vermes, I.

AU - van den Berg, Albert

N1 - Conference code: 12

PY - 2008/10/12

Y1 - 2008/10/12

N2 - Currently, the knowledge on breast cancer treatment is increasing, however, there are still hardly any assays present to match patients to the right form of therapy to enhance the therapeutic effectiveness. Therefore a microfluidic device is developed to analyze the hormonal and chemosensitivity of first breast cancer cell lines (MCF-7) and in a later stage a patients’ own tumor cells. The apoptotic inducers tumor ne-crosis factor-α, in combination with cycloheximide, and staurosporine stained MCF-7 cells positive for Annexin V and PI, and showed a decrease in area coverage as compared to untreated cells.

AB - Currently, the knowledge on breast cancer treatment is increasing, however, there are still hardly any assays present to match patients to the right form of therapy to enhance the therapeutic effectiveness. Therefore a microfluidic device is developed to analyze the hormonal and chemosensitivity of first breast cancer cell lines (MCF-7) and in a later stage a patients’ own tumor cells. The apoptotic inducers tumor ne-crosis factor-α, in combination with cycloheximide, and staurosporine stained MCF-7 cells positive for Annexin V and PI, and showed a decrease in area coverage as compared to untreated cells.

KW - EWI-14978

KW - METIS-256143

KW - IR-62715

M3 - Conference contribution

SN - 978-0-9798064-1-4

T3 - Micro Total Analysis Systems

SP - 549

EP - 551

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences

A2 - Locascio, L.E.

A2 - Gaitan, M.

A2 - Paegel, B.M.

A2 - Ross, D.J.

A2 - Vreeland, W.N.

PB - The Chemical and Biological Microsystems Society

CY - San Diego

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Wolbers F, Franke HR, Andersson H, Vermes I, Vermes I, van den Berg A. Microfluidic apoptosis chip for drug screening to improve and personalize cancer therapy. In Locascio LE, Gaitan M, Paegel BM, Ross DJ, Vreeland WN, editors, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences. San Diego: The Chemical and Biological Microsystems Society. 2008. p. 549-551. (Micro Total Analysis Systems).