Microfluidic DNA fragmentation for on-chip genomic analysis

Lingling Shui, Johan G. Bomer, Mingliang Jin, Edwin Carlen, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    17 Citations (Scopus)

    Abstract

    We report a high-throughput clog-free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Salmon sperm DNA has been reproducibly fragmented down to ∼5k bp fragment lengths by applying low hydraulic pressures (≤1 bar) across micromachined constrictions positioned in larger microfluidic channels that create point-sink flow with large velocity gradients near the constriction entrance. Long constrictions (100 μm) produce shorter fragment lengths compared to shorter constrictions (10 μm), while increasing the hydrodynamic pressure requirement. Sample recirculation (10×) in short constrictions reduces the mean fragment length and fragment length variation, and improves yield compared to single-pass experiments without increasing the hydrodynamic pressure.
    Original languageUndefined
    Pages (from-to)494013-494019
    Number of pages7
    JournalNanotechnology
    Volume22
    Issue number49
    DOIs
    Publication statusPublished - 9 Dec 2011

    Keywords

    • IR-78738
    • EWI-20924

    Cite this

    Shui, Lingling ; Bomer, Johan G. ; Jin, Mingliang ; Carlen, Edwin ; van den Berg, Albert. / Microfluidic DNA fragmentation for on-chip genomic analysis. In: Nanotechnology. 2011 ; Vol. 22, No. 49. pp. 494013-494019.
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    Microfluidic DNA fragmentation for on-chip genomic analysis. / Shui, Lingling; Bomer, Johan G.; Jin, Mingliang; Carlen, Edwin; van den Berg, Albert.

    In: Nanotechnology, Vol. 22, No. 49, 09.12.2011, p. 494013-494019.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Microfluidic DNA fragmentation for on-chip genomic analysis

    AU - Shui, Lingling

    AU - Bomer, Johan G.

    AU - Jin, Mingliang

    AU - Carlen, Edwin

    AU - van den Berg, Albert

    PY - 2011/12/9

    Y1 - 2011/12/9

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    AB - We report a high-throughput clog-free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Salmon sperm DNA has been reproducibly fragmented down to ∼5k bp fragment lengths by applying low hydraulic pressures (≤1 bar) across micromachined constrictions positioned in larger microfluidic channels that create point-sink flow with large velocity gradients near the constriction entrance. Long constrictions (100 μm) produce shorter fragment lengths compared to shorter constrictions (10 μm), while increasing the hydrodynamic pressure requirement. Sample recirculation (10×) in short constrictions reduces the mean fragment length and fragment length variation, and improves yield compared to single-pass experiments without increasing the hydrodynamic pressure.

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    KW - EWI-20924

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