Microfluidic Free-Flow Electrophoresis for Proteomics-on-a-Chip

D. Kohlheyer

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    74 Downloads (Pure)

    Abstract

    A new free-flow electrophoresis microchip with integrated permeable membranes was developed, and different substances were separated by free-flow zone electrophoresis, free-flow isoelectric focusing and free-flow field step electrophoresis. This chip contained a new type of membranes enabling a stable carrier flow with a perpendicular electrical current. Due to this chip configuration, the device performance and efficiency were superior to recently published alternative systems in terms of separation resolution and sample capacity. The results furthermore indicate that even better results are possible. Analytes were separated and focused within hundreds of milliseconds whereby only nanoliters of samples were consumed. In addition, a new sample steering method was demonstrated during free-flow zone electrophoresis, allowing the specific sorting of various components. As an alternative, a free-flow electrophoresis chip was developed with integrated platinum electrodes, whereby the generation of gas bubbles caused by electrolysis was successfully suppressed by chemical means. Gas bubbles generated by electrolysis are major concern in free-flow electrophoresis systems in general leading to distorted separation. Based on the results, a fourth free-flow chip was developed with an integrated surface plasmon resonance gold detection region. Although fabrication was successful, certain hurdles, in particular surface chemistry issues still remain to be overcome to perform separation and real-time detection of biological samples within this hyphenated micro device. A strategy for proteomics-on-a-chip was developed aiming at the separation of antigens that play a role in autoimmune diseases. In addition two new continuous flow microfluidic chips were developed allowing for continuous biochemical reactions of surface patterning applications. These devices could be of further interest in future, in particular in more complex analytical systems related to proteomics-on-a-chip.
    Original languageEnglish
    Awarding Institution
    • University of Twente
    Supervisors/Advisors
    • van den Berg, Albert , Supervisor
    • Schasfoort, R.B.M. , Advisor
    Thesis sponsors
    Award date20 Jun 2008
    Place of Publicationenschede
    Publisher
    Print ISBNs978-90-365-2666-1
    DOIs
    Publication statusPublished - 20 Jun 2008

    Fingerprint

    Electrophoresis
    Microfluidics
    Electrolysis
    Gases
    Membranes
    Surface plasmon resonance
    Platinum
    Surface chemistry
    Sorting
    Gold
    Proteomics
    Flow fields
    Antigens
    Fabrication
    Electrodes

    Keywords

    • EWI-14076
    • METIS-252107
    • IR-59014

    Cite this

    Kohlheyer, D.. / Microfluidic Free-Flow Electrophoresis for Proteomics-on-a-Chip. enschede : ippskamp, 2008. 186 p.
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    Microfluidic Free-Flow Electrophoresis for Proteomics-on-a-Chip. / Kohlheyer, D.

    enschede : ippskamp, 2008. 186 p.

    Research output: ThesisPhD Thesis - Research UT, graduation UT

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