Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applications

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademic


    Cell populations are heterogeneous: they can comprise different cell types or even cells at different stages of the cell cycle and/or of biological processes. Furthermore, molecular processes taking place in cells are stochastic in nature. Therefore, cellular analysis must be brought down to the single cell level to get useful insight into biological processes, and to access essential molecular information that would be lost when using a cell population analysis approach. Furthermore, to fully characterize a cell population, ideally, information both at the single cell level and on the whole cell population is required, which calls for analyzing each individual cell in a population in a parallel manner. This single cell level analysis approach is particularly important for diagnostic applications to unravel molecular perturbations at the onset of a disease, to identify biomarkers, and for personalized medicine, not only because of the heterogeneity of the cell sample, but also due to the availability of a reduced amount of cells, or even unique cells. This chapter presents a versatile platform meant for the parallel analysis of individual cells, with a particular focus on diagnostic applications and the analysis of cancer cells. We first describe one essential step of this parallel single cell analysis protocol, which is the trapping of individual cells in dedicated structures. Following this, we report different steps of a whole analytical process, including on-chip cell staining and imaging, cell membrane permeabilization and/or lysis using either chemical or physical means, and retrieval of the cell molecular content in dedicated channels for further analysis. This series of experiments illustrates the versatility of the herein-presented platform and its suitability for various analysis schemes and different analytical purposes.
    Original languageEnglish
    Title of host publicationMicrochip Diagnostics
    EditorsValerie Taly, Stephanie Descroix, Jean-Louis Viovy
    ISBN (Electronic)978-1-4939-6734-6
    ISBN (Print)978-1-4939-6732-2
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    PublisherSpringer Science+Business Media LCC
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029


    • Single cell
    • Parallelization
    • Microfluidics
    • Circulating tumor cells
    • Cell trapping
    • Cell permeabilization


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