Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering

Björn Harink; Séverine le Gac; Clemens van Blitterswijk; Pamela Habibovic

Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering

Björn Harink, Séverine le Gac, Clemens van Blitterswijk, Pamela Habibovic

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We propose a microfluidics-based cell-culture platform for the screening of trace elements in bone tissue engineering. With this platform, it is possible to create four stable, independent and perpendicular diffusion-based concentration gradients in a single square chamber, in which cells are cultured. This allows examining the combined effect of four compounds in a single assay.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Editors	Sabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme
Publisher	The Chemical and Biological Microsystems Society
Pages	638-640
ISBN (Print)	978-0-9798064-3-8
Publication status	Published - 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14

Publication series

Name	International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
Publisher	The Chemical and Biological Microsystems Society
Volume	2010
ISSN (Print)	1556-5904

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
Abbreviated title	MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Keywords

Gradient
Trace element
Bone
Tissue engineering

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Harink2010microfluidicFinal published version, 378 KB

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Harink, B., le Gac, S., van Blitterswijk, C., & Habibovic, P. (2010). Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering. In S. Verpoorte, H. Andersson-Svahn, J. Emnéus, & N. Pamme (Eds.), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 638-640). (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010). The Chemical and Biological Microsystems Society.

Harink, Björn ; le Gac, Séverine ; van Blitterswijk, Clemens et al. / Microfluidic platform for the simultaneous generation of four independent gradients : towards the high throughput screening of trace elements for bone tissue engineering. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. editor / Sabeth Verpoorte ; Helen Andersson-Svahn ; Jenny Emnéus ; Nicole Pamme. The Chemical and Biological Microsystems Society, 2010. pp. 638-640 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

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title = "Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering",

abstract = "We propose a microfluidics-based cell-culture platform for the screening of trace elements in bone tissue engineering. With this platform, it is possible to create four stable, independent and perpendicular diffusion-based concentration gradients in a single square chamber, in which cells are cultured. This allows examining the combined effect of four compounds in a single assay.",

keywords = "Gradient, Trace element, Bone, Tissue engineering",

author = "Bj{\"o}rn Harink and {le Gac}, S{\'e}verine and {van Blitterswijk}, Clemens and Pamela Habibovic",

year = "2010",

language = "English",

isbn = "978-0-9798064-3-8",

series = "International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]",

publisher = "The Chemical and Biological Microsystems Society",

pages = "638--640",

editor = "Sabeth Verpoorte and Helen Andersson-Svahn and Jenny Emn{\'e}us and Nicole Pamme",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",

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Harink, B, le Gac, S, van Blitterswijk, C & Habibovic, P 2010, Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering. in S Verpoorte, H Andersson-Svahn, J Emnéus & N Pamme (eds), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings], vol. 2010, The Chemical and Biological Microsystems Society, pp. 638-640, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, Groningen, Netherlands, 3/10/10.

Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering. / Harink, Björn; le Gac, Séverine; van Blitterswijk, Clemens et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. ed. / Sabeth Verpoorte; Helen Andersson-Svahn; Jenny Emnéus; Nicole Pamme. The Chemical and Biological Microsystems Society, 2010. p. 638-640 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Microfluidic platform for the simultaneous generation of four independent gradients

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

AU - Harink, Björn

AU - le Gac, Séverine

AU - van Blitterswijk, Clemens

AU - Habibovic, Pamela

N1 - Conference code: 14

PY - 2010

Y1 - 2010

N2 - We propose a microfluidics-based cell-culture platform for the screening of trace elements in bone tissue engineering. With this platform, it is possible to create four stable, independent and perpendicular diffusion-based concentration gradients in a single square chamber, in which cells are cultured. This allows examining the combined effect of four compounds in a single assay.

AB - We propose a microfluidics-based cell-culture platform for the screening of trace elements in bone tissue engineering. With this platform, it is possible to create four stable, independent and perpendicular diffusion-based concentration gradients in a single square chamber, in which cells are cultured. This allows examining the combined effect of four compounds in a single assay.

KW - Gradient

KW - Trace element

KW - Bone

KW - Tissue engineering

M3 - Conference contribution

SN - 978-0-9798064-3-8

T3 - International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]

SP - 638

EP - 640

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

A2 - Verpoorte, Sabeth

A2 - Andersson-Svahn, Helen

A2 - Emnéus, Jenny

A2 - Pamme, Nicole

PB - The Chemical and Biological Microsystems Society

Y2 - 3 October 2010 through 7 October 2010

ER -

Harink B, le Gac S, van Blitterswijk C, Habibovic P. Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering. In Verpoorte S, Andersson-Svahn H, Emnéus J, Pamme N, editors, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. The Chemical and Biological Microsystems Society. 2010. p. 638-640. (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

Microfluidic platform for the simultaneous generation of four independent gradients: towards the high throughput screening of trace elements for bone tissue engineering

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