Microfluidic protocol for in vitro culture of human embryos

Zhenxia Hao, D.C. Kieslinger, C. Vergouw, H. Kostelijk, C.B. Lambalk, Severine le Gac

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 & 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.
    Original languageUndefined
    Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
    EditorsR. Zengerle
    Place of PublicationFreiburg
    PublisherThe Printing House
    Pages536-538
    Number of pages3
    ISBN (Print)978-0-9798064-6-9
    Publication statusPublished - 27 Oct 2013
    Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany
    Duration: 27 Oct 201331 Oct 2013
    Conference number: 17

    Publication series

    NameMicroTAS
    PublisherThe Printing House
    NumberMicroTAS 2013
    ISSN (Print)1556-5904

    Conference

    Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
    Abbreviated titleMicroTAS
    CountryGermany
    CityFreiburg
    Period27/10/1331/10/13

    Keywords

    • EWI-24052
    • IR-88174
    • METIS-300197

    Cite this

    Hao, Z., Kieslinger, D. C., Vergouw, C., Kostelijk, H., Lambalk, C. B., & le Gac, S. (2013). Microfluidic protocol for in vitro culture of human embryos. In R. Zengerle (Ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS (pp. 536-538). (MicroTAS; No. MicroTAS 2013). Freiburg: The Printing House.