Microfluidic protocol for in vitro culture of human embryos

Zhenxia Hao; D.C. Kieslinger; C. Vergouw; H. Kostelijk; C.B. Lambalk; Severine le Gac

Microfluidic protocol for in vitro culture of human embryos

Zhenxia Hao, D.C. Kieslinger, C. Vergouw, H. Kostelijk, C.B. Lambalk, Severine le Gac

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 & 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.

Original language	Undefined
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Editors	R. Zengerle
Place of Publication	Freiburg
Publisher	The Printing House
Pages	536-538
Number of pages	3
ISBN (Print)	978-0-9798064-6-9
Publication status	Published - 27 Oct 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 Conference number: 17

Publication series

Name	MicroTAS
Publisher	The Printing House
Number	MicroTAS 2013
ISSN (Print)	1556-5904

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
Abbreviated title	MicroTAS
Country/Territory	Germany
City	Freiburg
Period	27/10/13 → 31/10/13

Keywords

EWI-24052
IR-88174
METIS-300197

Access to Document

http://eprints.eemcs.utwente.nl/secure2/24052/02/180_0208.pdf

Cite this

Hao, Z., Kieslinger, D. C., Vergouw, C., Kostelijk, H., Lambalk, C. B., & le Gac, S. (2013). Microfluidic protocol for in vitro culture of human embryos. In R. Zengerle (Ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS (pp. 536-538). (MicroTAS; No. MicroTAS 2013). The Printing House. http://eprints.eemcs.utwente.nl/secure2/24052/02/180_0208.pdf

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title = "Microfluidic protocol for in vitro culture of human embryos",

abstract = "In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 \& 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.",

keywords = "EWI-24052, IR-88174, METIS-300197",

author = "Zhenxia Hao and D.C. Kieslinger and C. Vergouw and H. Kostelijk and C.B. Lambalk and \{le Gac\}, Severine",

year = "2013",

month = oct,

day = "27",

language = "Undefined",

isbn = "978-0-9798064-6-9",

series = "MicroTAS",

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number = "MicroTAS 2013",

pages = "536--538",

editor = "R. Zengerle",

booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

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Hao, Z, Kieslinger, DC, Vergouw, C, Kostelijk, H, Lambalk, CB & le Gac, S 2013, Microfluidic protocol for in vitro culture of human embryos. in R Zengerle (ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. MicroTAS, no. MicroTAS 2013, The Printing House, Freiburg, pp. 536-538, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013, Freiburg, Germany, 27/10/13. <http://eprints.eemcs.utwente.nl/secure2/24052/02/180_0208.pdf>

Microfluidic protocol for in vitro culture of human embryos. / Hao, Zhenxia; Kieslinger, D.C.; Vergouw, C. et al.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. ed. / R. Zengerle. Freiburg: The Printing House, 2013. p. 536-538 (MicroTAS; No. MicroTAS 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - Microfluidic protocol for in vitro culture of human embryos

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AU - le Gac, Severine

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N2 - In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 & 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.

AB - In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 & 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.

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BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

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