Microfluidic single sperm entrapment and analysis

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy
Original languageUndefined
Pages (from-to)1294-1301
Number of pages8
JournalLab on a chip
Volume15
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • EWI-26006
  • METIS-312598
  • IR-95879

Cite this

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title = "Microfluidic single sperm entrapment and analysis",
abstract = "Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy",
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author = "{de Wagenaar}, B. and Berendsen, {Johanna Theodora Wilhelmina} and J.T.W. Berendsen and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert and Segerink, {Loes Irene}",
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doi = "10.1039/c4lc01425a",
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volume = "15",
pages = "1294--1301",
journal = "Lab on a chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
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Microfluidic single sperm entrapment and analysis. / de Wagenaar, B.; Berendsen, Johanna Theodora Wilhelmina; Berendsen, J.T.W.; Bomer, Johan G.; Olthuis, Wouter; van den Berg, Albert; Segerink, Loes Irene.

In: Lab on a chip, Vol. 15, No. 5, 2015, p. 1294-1301.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Microfluidic single sperm entrapment and analysis

AU - de Wagenaar, B.

AU - Berendsen, Johanna Theodora Wilhelmina

AU - Berendsen, J.T.W.

AU - Bomer, Johan G.

AU - Olthuis, Wouter

AU - van den Berg, Albert

AU - Segerink, Loes Irene

N1 - eemcs-eprint-26006

PY - 2015

Y1 - 2015

N2 - Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy

AB - Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy

KW - EWI-26006

KW - METIS-312598

KW - IR-95879

U2 - 10.1039/c4lc01425a

DO - 10.1039/c4lc01425a

M3 - Article

VL - 15

SP - 1294

EP - 1301

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 5

ER -