Microfluidic single sperm entrapment and analysis

B. de Wagenaar, Johanna Theodora Wilhelmina Berendsen, J.T.W. Berendsen, Johan G. Bomer, Wouter Olthuis, Albert van den Berg, Loes Irene Segerink

    Research output: Contribution to journalArticleAcademicpeer-review

    24 Citations (Scopus)
    2 Downloads (Pure)

    Abstract

    Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy
    Original languageUndefined
    Pages (from-to)1294-1301
    Number of pages8
    JournalLab on a chip
    Volume15
    Issue number5
    DOIs
    Publication statusPublished - 2015

    Keywords

    • EWI-26006
    • METIS-312598
    • IR-95879

    Cite this

    de Wagenaar, B. ; Berendsen, Johanna Theodora Wilhelmina ; Berendsen, J.T.W. ; Bomer, Johan G. ; Olthuis, Wouter ; van den Berg, Albert ; Segerink, Loes Irene. / Microfluidic single sperm entrapment and analysis. In: Lab on a chip. 2015 ; Vol. 15, No. 5. pp. 1294-1301.
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    title = "Microfluidic single sperm entrapment and analysis",
    abstract = "Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy",
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    author = "{de Wagenaar}, B. and Berendsen, {Johanna Theodora Wilhelmina} and J.T.W. Berendsen and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert and Segerink, {Loes Irene}",
    note = "eemcs-eprint-26006",
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    doi = "10.1039/c4lc01425a",
    language = "Undefined",
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    Microfluidic single sperm entrapment and analysis. / de Wagenaar, B.; Berendsen, Johanna Theodora Wilhelmina; Berendsen, J.T.W.; Bomer, Johan G.; Olthuis, Wouter; van den Berg, Albert; Segerink, Loes Irene.

    In: Lab on a chip, Vol. 15, No. 5, 2015, p. 1294-1301.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Microfluidic single sperm entrapment and analysis

    AU - de Wagenaar, B.

    AU - Berendsen, Johanna Theodora Wilhelmina

    AU - Berendsen, J.T.W.

    AU - Bomer, Johan G.

    AU - Olthuis, Wouter

    AU - van den Berg, Albert

    AU - Segerink, Loes Irene

    N1 - eemcs-eprint-26006

    PY - 2015

    Y1 - 2015

    N2 - Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy

    AB - Selection of healthy spermatozoa is of crucial importance for the success rates of assisted reproduction technologies (ART) such as in vitro fertilization and intra-cytoplasmic sperm injection. Although sperm selection for ART procedures is predominantly based on sperm motility, successful fertilization is not predicted by good motility alone. For example, sperm characteristics such as the acrosome state and DNA integrity have shown significant impact on ART outcome. Although fertilization can be achieved with a single spermatozoon of high quality, current quality assessments are population-based and do not allow investigation of multiple sperm characteristics on a single spermatozoon simultaneously. In order to study sperm cells on the single cell level, we designed and characterized a PDMS microfluidic platform that allows single sperm entrapment. After spatially confining individual sperm cells within microfluidic cell traps, the cell viability, chromosomal content and acrosome state were studied. This platform is suitable for the analysis of individual sperm cells, which could be exploited for (non-invasive) sperm analysis and selection by impedance or Raman spectroscopy

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    KW - METIS-312598

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