Abstract
Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy
rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages,
before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop
faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.
Original language | English |
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Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 |
Editors | Sabeth Verpoorte, Helen Andersson-Svath, Jenny Emnéus, Nicole Pamme |
Place of Publication | San Diego |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 1484-1486 |
Number of pages | 3 |
ISBN (Electronic) | 978-0-9798064-3-8 |
ISBN (Print) | 978-1-6183906-2-2 |
Publication status | Published - 3 Oct 2010 |
Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14 |
Publication series
Name | International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings] |
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Publisher | The Chemical and Biological Microsystems Society |
Volume | 2010 |
ISSN (Print) | 1556-5904 |
Conference
Conference | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 |
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Abbreviated title | MicroTAS 2010 |
Country/Territory | Netherlands |
City | Groningen |
Period | 3/10/10 → 7/10/10 |
Keywords
- Assisted reproductive technologies
- Embryo culture
- Micro-fluidics
- Mammalian embryo