Microfluidic systems for improving assisted reproductive technologies culture protocols

F. van Rossem; T.C. Esteves; M. Boiani; S. le Gac; A. van den Berg

Microfluidic systems for improving assisted reproductive technologies culture protocols

F. van Rossem, T.C. Esteves, M. Boiani, S. le Gac, A. van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

11 Downloads (Pure)

Abstract

Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
Editors	Sabeth Verpoorte, Helen Andersson-Svath, Jenny Emnéus, Nicole Pamme
Place of Publication	San Diego
Publisher	The Chemical and Biological Microsystems Society
Pages	1484-1486
Number of pages	3
ISBN (Electronic)	978-0-9798064-3-8
ISBN (Print)	978-1-6183906-2-2
Publication status	Published - 3 Oct 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14

Publication series

Name	International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
Publisher	The Chemical and Biological Microsystems Society
Volume	2010
ISSN (Print)	1556-5904

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
Abbreviated title	MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Keywords

Assisted reproductive technologies
Embryo culture
Micro-fluidics
Mammalian embryo

Access to Document

VanRossem2010microfluidicFinal published version, 1.2 MB

Cite this

van Rossem, F., Esteves, T. C., Boiani, M., le Gac, S., & van den Berg, A. (2010). Microfluidic systems for improving assisted reproductive technologies culture protocols. In S. Verpoorte, H. Andersson-Svath, J. Emnéus, & N. Pamme (Eds.), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 (pp. 1484-1486). (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010). The Chemical and Biological Microsystems Society.

van Rossem, F. ; Esteves, T.C. ; Boiani, M. et al. / Microfluidic systems for improving assisted reproductive technologies culture protocols. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. editor / Sabeth Verpoorte ; Helen Andersson-Svath ; Jenny Emnéus ; Nicole Pamme. San Diego : The Chemical and Biological Microsystems Society, 2010. pp. 1484-1486 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

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title = "Microfluidic systems for improving assisted reproductive technologies culture protocols",

abstract = "Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.",

keywords = "Assisted reproductive technologies, Embryo culture, Micro-fluidics, Mammalian embryo",

author = "{van Rossem}, F. and T.C. Esteves and M. Boiani and {le Gac}, S. and {van den Berg}, A.",

year = "2010",

month = oct,

day = "3",

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isbn = "978-1-6183906-2-2",

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editor = "Sabeth Verpoorte and Helen Andersson-Svath and Jenny Emn{\'e}us and Nicole Pamme",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010",

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van Rossem, F, Esteves, TC, Boiani, M, le Gac, S & van den Berg, A 2010, Microfluidic systems for improving assisted reproductive technologies culture protocols. in S Verpoorte, H Andersson-Svath, J Emnéus & N Pamme (eds), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings], vol. 2010, The Chemical and Biological Microsystems Society, San Diego, pp. 1484-1486, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, Groningen, Netherlands, 3/10/10.

Microfluidic systems for improving assisted reproductive technologies culture protocols. / van Rossem, F.; Esteves, T.C.; Boiani, M. et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. ed. / Sabeth Verpoorte; Helen Andersson-Svath; Jenny Emnéus; Nicole Pamme. San Diego: The Chemical and Biological Microsystems Society, 2010. p. 1484-1486 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Microfluidic systems for improving assisted reproductive technologies culture protocols

AU - van Rossem, F.

AU - Esteves, T.C.

AU - Boiani, M.

AU - le Gac, S.

AU - van den Berg, A.

N1 - Conference code: 14

PY - 2010/10/3

Y1 - 2010/10/3

N2 - Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.

AB - Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.

KW - Assisted reproductive technologies

KW - Embryo culture

KW - Micro-fluidics

KW - Mammalian embryo

M3 - Conference contribution

SN - 978-1-6183906-2-2

T3 - International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]

SP - 1484

EP - 1486

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

A2 - Verpoorte, Sabeth

A2 - Andersson-Svath, Helen

A2 - Emnéus, Jenny

A2 - Pamme, Nicole

PB - The Chemical and Biological Microsystems Society

CY - San Diego

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

van Rossem F, Esteves TC, Boiani M, le Gac S , van den Berg A. Microfluidic systems for improving assisted reproductive technologies culture protocols. In Verpoorte S, Andersson-Svath H, Emnéus J, Pamme N, editors, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. San Diego: The Chemical and Biological Microsystems Society. 2010. p. 1484-1486. (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).