Microfluidic systems for improving assisted reproductive technologies culture protocols

F. van Rossem, T.C. Esteves, M. Boiani, S. le Gac, A. van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)

Abstract

Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.
Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
EditorsSabeth Verpoorte, Helen Andersson-Svath, Jenny Emnéus, Nicole Pamme
Place of PublicationSan Diego
PublisherChemical and Biological Micro Systems Society
Pages1484-1486
Number of pages3
ISBN (Electronic)978-0-9798064-3-8
ISBN (Print)978-1-6183906-2-2
Publication statusPublished - 3 Oct 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010
Conference number: 14

Publication series

NameInternational Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
PublisherThe Chemical and Biological Microsystems Society
Volume2010
ISSN (Print)1556-5904

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010
Abbreviated titleMicroTAS
CountryNetherlands
CityGroningen
Period3/10/107/10/10

Fingerprint

assisted reproductive technologies
embryo (animal)
embryo culture
mice
blastocyst
pups
embryogenesis

Keywords

  • Assisted reproductive technologies
  • Embryo culture
  • Micro-fluidics
  • Mammalian embryo

Cite this

van Rossem, F., Esteves, T. C., Boiani, M., le Gac, S., & van den Berg, A. (2010). Microfluidic systems for improving assisted reproductive technologies culture protocols. In S. Verpoorte, H. Andersson-Svath, J. Emnéus, & N. Pamme (Eds.), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 (pp. 1484-1486). (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010). San Diego: Chemical and Biological Micro Systems Society.
van Rossem, F. ; Esteves, T.C. ; Boiani, M. ; le Gac, S. ; van den Berg, A. / Microfluidic systems for improving assisted reproductive technologies culture protocols. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. editor / Sabeth Verpoorte ; Helen Andersson-Svath ; Jenny Emnéus ; Nicole Pamme. San Diego : Chemical and Biological Micro Systems Society, 2010. pp. 1484-1486 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).
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title = "Microfluidic systems for improving assisted reproductive technologies culture protocols",
abstract = "Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30{\%}). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.",
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van Rossem, F, Esteves, TC, Boiani, M, le Gac, S & van den Berg, A 2010, Microfluidic systems for improving assisted reproductive technologies culture protocols. in S Verpoorte, H Andersson-Svath, J Emnéus & N Pamme (eds), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings], vol. 2010, Chemical and Biological Micro Systems Society, San Diego, pp. 1484-1486, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010, Groningen, Netherlands, 3/10/10.

Microfluidic systems for improving assisted reproductive technologies culture protocols. / van Rossem, F.; Esteves, T.C.; Boiani, M.; le Gac, S.; van den Berg, A.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. ed. / Sabeth Verpoorte; Helen Andersson-Svath; Jenny Emnéus; Nicole Pamme. San Diego : Chemical and Biological Micro Systems Society, 2010. p. 1484-1486 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Microfluidic systems for improving assisted reproductive technologies culture protocols

AU - van Rossem, F.

AU - Esteves, T.C.

AU - Boiani, M.

AU - le Gac, S.

AU - van den Berg, A.

PY - 2010/10/3

Y1 - 2010/10/3

N2 - Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.

AB - Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.

KW - Assisted reproductive technologies

KW - Embryo culture

KW - Micro-fluidics

KW - Mammalian embryo

M3 - Conference contribution

SN - 978-1-6183906-2-2

T3 - International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]

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BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

A2 - Verpoorte, Sabeth

A2 - Andersson-Svath, Helen

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PB - Chemical and Biological Micro Systems Society

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van Rossem F, Esteves TC, Boiani M, le Gac S, van den Berg A. Microfluidic systems for improving assisted reproductive technologies culture protocols. In Verpoorte S, Andersson-Svath H, Emnéus J, Pamme N, editors, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. San Diego: Chemical and Biological Micro Systems Society. 2010. p. 1484-1486. (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).