Microfluidic valves with integrated structured elastomeric membranes for reversible fluidic entrapment and in situ channel functionalization

We report the utility Of Structured elastomeric membranes (SEMs) its it multifunctional microfluidic These Structured membranes are part of a two-layer microfluidic device. analogous to membrane tool. valves, with the novelty that they incorporate topographical features oil the roof of the fluid channel. We demonstrate that when the topographical features are recessed into the roof of the fluid channel, actuation of the membrane leads to effective confinement of fluids down to femtolitres in Preformed microfluidic containers. Thus, the SEMs in this case function its fluidic traps that Could be Coupled to microfluidic networks for rapid and repeated flushing of solvents. Alternatively. when the topographical features oil the roof protrude into the fluid channel, we demonstrate that the SEMs call be used to pattern proteins and cells in microchannels. Thus ill this case, the SEMs serve its fluidic stamps for functionalizing microchannel surfaces. In addition. we show that the trap or pattern shape and size call be manipulated simply by varying the topography oil the elastomeric membrane. Since SEW membrane valves and Pumps use similar fabrication technology, we believe that SEMs call be integrated into microfluidic large-scale circuits for biotechnological applications.