Micromachined heated chemical reactor for pre-column derivatisation

Jan C.T. Eijkel, Albert Prak, Simon Cowen, Derek H. Craston, Andreas Manz*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)


A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 μl volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2°C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC. As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60°C, followed by HPLC separation and fluorescence detection.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalJournal of chromatography A
Issue number2
Publication statusPublished - 31 Jul 1998
Externally publishedYes


  • 4-Fluoro-7-nitrobenzofuran
  • Amino acids
  • Chip technology
  • Derivatization, LC
  • Micro-total analysis system
  • Microreactor
  • Sodium azide


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