Micromachined heated chemical reactor for pre-column derivatisation

Jan C.T. Eijkel; Albert Prak; Simon Cowen; Derek H. Craston; Andreas Manz

doi:10.1016/S0021-9673(98)00459-2

Micromachined heated chemical reactor for pre-column derivatisation

Jan C.T. Eijkel, Albert Prak, Simon Cowen, Derek H. Craston, Andreas Manz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

26 Citations (Scopus)

Abstract

A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 μl volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2°C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC. As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60°C, followed by HPLC separation and fluorescence detection.

Original language	English
Pages (from-to)	265-271
Number of pages	7
Journal	Journal of chromatography A
Volume	815
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(98)00459-2
Publication status	Published - 31 Jul 1998
Externally published	Yes

Keywords

4-Fluoro-7-nitrobenzofuran
Amino acids
Chip technology
Derivatization, LC
Micro-total analysis system
Microreactor
Sodium azide

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10.1016/S0021-9673(98)00459-2

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title = "Micromachined heated chemical reactor for pre-column derivatisation",

abstract = "A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 μl volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2°C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC. As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60°C, followed by HPLC separation and fluorescence detection. ",

keywords = "4-Fluoro-7-nitrobenzofuran, Amino acids, Chip technology, Derivatization, LC, Micro-total analysis system, Microreactor, Sodium azide",

author = "Eijkel, \{Jan C.T.\} and Albert Prak and Simon Cowen and Craston, \{Derek H.\} and Andreas Manz",

year = "1998",

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day = "31",

doi = "10.1016/S0021-9673(98)00459-2",

language = "English",

volume = "815",

pages = "265--271",

journal = "Journal of chromatography A",

issn = "0021-9673",

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TY - JOUR

T1 - Micromachined heated chemical reactor for pre-column derivatisation

AU - Eijkel, Jan C.T.

AU - Prak, Albert

AU - Cowen, Simon

AU - Craston, Derek H.

AU - Manz, Andreas

PY - 1998/7/31

Y1 - 1998/7/31

N2 - A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 μl volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2°C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC. As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60°C, followed by HPLC separation and fluorescence detection.

AB - A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 μl volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2°C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC. As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60°C, followed by HPLC separation and fluorescence detection.

KW - 4-Fluoro-7-nitrobenzofuran

KW - Amino acids

KW - Chip technology

KW - Derivatization, LC

KW - Micro-total analysis system

KW - Microreactor

KW - Sodium azide

UR - https://www.scopus.com/pages/publications/0031830518

U2 - 10.1016/S0021-9673(98)00459-2

DO - 10.1016/S0021-9673(98)00459-2

M3 - Article

AN - SCOPUS:0031830518

SN - 0021-9673

VL - 815

SP - 265

EP - 271

JO - Journal of chromatography A

JF - Journal of chromatography A

IS - 2

ER -

Micromachined heated chemical reactor for pre-column derivatisation

Abstract

Keywords

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