We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.
|Title of host publication||Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912|
|Editors||Raymond P. Mariella Jr.|
|Place of Publication||Bellingham, WA, USA|
|Number of pages||9|
|Publication status||Published - 26 Jan 2000|
|Name||Proceedings of SPIE|