Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

C.R. Rusu, Ronny van 't Oever, Meint J. de Boer, Johan W. Berenschot, Michael Curt Elwenspoek, Henricus V. Jansen, Martin L. Bennink, Johannes S. Kanger, B.G. de Grooth, Jan Greve, J.P. Brugger, Albert van den Berg, M. de Boer

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.
Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering, Vol. 3912
EditorsRaymond P. Mariella Jr.
Place of PublicationBellingham, WA, USA
PublisherSPIE
Pages41-49
Number of pages9
ISBN (Print)0-8194-3528-7
DOIs
Publication statusPublished - 26 Jan 2000

Publication series

NameProceedings of SPIE
PublisherSPIE
Number6
Volume1

Keywords

  • EWI-13187
  • IR-37010
  • METIS-203699

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  • Cite this

    Rusu, C. R., van 't Oever, R., de Boer, M. J., Berenschot, J. W., Elwenspoek, M. C., Jansen, H. V., ... de Boer, M. (2000). Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping. In R. P. Mariella Jr. (Ed.), Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912 (pp. 41-49). (Proceedings of SPIE; Vol. 1, No. 6). Bellingham, WA, USA: SPIE. https://doi.org/10.1117/12.379579