Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

Cristina Rusu; Ronny van 't Oever; Meint de Boer; Henri Jansen; Erwin Berenschot; Miko Elwenspoek; Martin L. Bennink; Johannes S. Kanger; Bart G. de Grooth; Jan Greve; Jürgen Brugger; Albert van den Berg

doi:10.1117/12.379579

Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

Cristina Rusu
, Ronny van 't Oever
, Meint de Boer
, Henri Jansen
, Erwin Berenschot
, Miko Elwenspoek
, Martin L. Bennink
, Johannes S. Kanger
, Bart G. de Grooth
, Jan Greve
, Jürgen Brugger
, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912
Editors	Raymond P. Mariella Jr.
Place of Publication	Bellingham, WA, USA
Publisher	SPIE
Pages	41-49
Number of pages	9
ISBN (Print)	0-8194-3528-7
DOIs	https://doi.org/10.1117/12.379579
Publication status	Published - 26 Jan 2000
Event	Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912 - San Jose, CA, USA Duration: 26 Jan 2000 → 27 Jan 2000

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	3912

Conference

Conference	Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912
Period	26/01/00 → 27/01/00
Other	26-27 Jan 2000

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Keywords

2025 OA procedure

Access to Document

10.1117/12.379579

ArticleFinal published version, 2.97 MBLicence: Taverne

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Rusu, C., van 't Oever, R., de Boer, M., Jansen, H., Berenschot, E., Elwenspoek, M., Bennink, M. L., Kanger, J. S., de Grooth, B. G., Greve, J., Brugger, J., & van den Berg, A. (2000). Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping. In R. P. Mariella Jr. (Ed.), Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912 (pp. 41-49). (Proceedings of SPIE; Vol. 3912). SPIE. https://doi.org/10.1117/12.379579

@inproceedings{261d55f5f19244e9a90da9eacb4a11ef,

title = "Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping",

abstract = "We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.",

keywords = "2025 OA procedure",

author = "Cristina Rusu and \{van 't Oever\}, Ronny and \{de Boer\}, Meint and Henri Jansen and Erwin Berenschot and Miko Elwenspoek and Bennink, \{Martin L.\} and Kanger, \{Johannes S.\} and \{de Grooth\}, \{Bart G.\} and Jan Greve and J{\"u}rgen Brugger and \{van den Berg\}, Albert",

year = "2000",

month = jan,

day = "26",

doi = "10.1117/12.379579",

language = "English",

isbn = "0-8194-3528-7",

series = "Proceedings of SPIE",

publisher = "SPIE",

pages = "41--49",

editor = "\{Mariella Jr.\}, \{Raymond P.\}",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912",

address = "United States",

note = "Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912 ; Conference date: 26-01-2000 Through 27-01-2000",

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Rusu, C, van 't Oever, R, de Boer, M, Jansen, H, Berenschot, E, Elwenspoek, M, Bennink, ML , Kanger, JS, de Grooth, BG, Greve, J, Brugger, J & van den Berg, A 2000, Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping. in RP Mariella Jr. (ed.), Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912. Proceedings of SPIE, vol. 3912, SPIE, Bellingham, WA, USA, pp. 41-49, Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912, 26/01/00. https://doi.org/10.1117/12.379579

Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping. / Rusu, Cristina; van 't Oever, Ronny; de Boer, Meint et al.
Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912. ed. / Raymond P. Mariella Jr. Bellingham, WA, USA: SPIE, 2000. p. 41-49 (Proceedings of SPIE; Vol. 3912).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

AU - Rusu, Cristina

AU - van 't Oever, Ronny

AU - de Boer, Meint

AU - Jansen, Henri

AU - Berenschot, Erwin

AU - Elwenspoek, Miko

AU - Bennink, Martin L.

AU - Kanger, Johannes S.

AU - de Grooth, Bart G.

AU - Greve, Jan

AU - Brugger, Jürgen

AU - van den Berg, Albert

PY - 2000/1/26

Y1 - 2000/1/26

N2 - We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.

AB - We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap set-up (optical tweezers) to study mechanical and structural properties of λ-DNA molecules. The flow cell was realized using silicon micromachining including the so-called buried channel technology to fabricate the micropipettes, the wet etching of glass to create the flow channel, and the powder blasting of glass to create the fluid connections. The volume of the flow cell is 2 µl. The pipettes have a length of 130 µm, a width of 5-10 µm, a round opening of 1 micron and can be processed with different shapes. Using this flow cell we stretched single molecules (λ-DNA) showing typical force-extension curves also found with conventional techniques.

KW - 2025 OA procedure

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DO - 10.1117/12.379579

M3 - Conference contribution

SN - 0-8194-3528-7

T3 - Proceedings of SPIE

SP - 41

EP - 49

BT - Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912

A2 - Mariella Jr., Raymond P.

PB - SPIE

CY - Bellingham, WA, USA

T2 - Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912

Y2 - 26 January 2000 through 27 January 2000

ER -

Rusu C, van 't Oever R, de Boer M, Jansen H, Berenschot E, Elwenspoek M et al. Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping. In Mariella Jr. RP, editor, Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3912. Bellingham, WA, USA: SPIE. 2000. p. 41-49. (Proceedings of SPIE). doi: 10.1117/12.379579

Micromachined pipettes integrated in a flow channel for single DNA molecule study by optical trapping

Abstract

Publication series

Conference

UN SDGs

Keywords

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