Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils

Mieke M.J.F. Koenders, Lanti Yang, Ronnie G. Wismans, Kees van der Werf, Dieter P. Reinhardt, Willeke Daamen, Martin L. Bennink, Pieter J. Dijkstra, Toin H. van Kuppevelt, Jan Feijen

Research output: Contribution to journalArticleAcademic

23 Citations (Scopus)

Abstract

Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.
Original languageEnglish
Pages (from-to)2425-2432
JournalBiomaterials
Volume30
Issue number13
DOIs
Publication statusPublished - 2009

Fingerprint

Microfibrils
Elastic Tissue
Elastic Modulus
Mechanical properties
Fibers
Elastic moduli
Atomic Force Microscopy
Vertebrates
Atomic force microscopy
Bending tests
Nanoindentation
Indentation
Horses
Fibrillins
Water
Substrates

Keywords

  • IR-60757
  • METIS-262783
  • AFM (atomic force microscopy)
  • Elastin
  • Mechanical properties
  • Mechanical test
  • Nano-indentation

Cite this

Koenders, M. M. J. F., Yang, L., Wismans, R. G., van der Werf, K., Reinhardt, D. P., Daamen, W., ... Feijen, J. (2009). Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils. Biomaterials, 30(13), 2425-2432. https://doi.org/10.1016/j.biomaterials.2009.01.038
Koenders, Mieke M.J.F. ; Yang, Lanti ; Wismans, Ronnie G. ; van der Werf, Kees ; Reinhardt, Dieter P. ; Daamen, Willeke ; Bennink, Martin L. ; Dijkstra, Pieter J. ; van Kuppevelt, Toin H. ; Feijen, Jan. / Microscale mechanical properties of single elastic fibers : The role of fibrillin-microfibrils. In: Biomaterials. 2009 ; Vol. 30, No. 13. pp. 2425-2432.
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abstract = "Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.",
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author = "Koenders, {Mieke M.J.F.} and Lanti Yang and Wismans, {Ronnie G.} and {van der Werf}, Kees and Reinhardt, {Dieter P.} and Willeke Daamen and Bennink, {Martin L.} and Dijkstra, {Pieter J.} and {van Kuppevelt}, {Toin H.} and Jan Feijen",
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Koenders, MMJF, Yang, L, Wismans, RG, van der Werf, K, Reinhardt, DP, Daamen, W, Bennink, ML, Dijkstra, PJ, van Kuppevelt, TH & Feijen, J 2009, 'Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils' Biomaterials, vol. 30, no. 13, pp. 2425-2432. https://doi.org/10.1016/j.biomaterials.2009.01.038

Microscale mechanical properties of single elastic fibers : The role of fibrillin-microfibrils. / Koenders, Mieke M.J.F.; Yang, Lanti; Wismans, Ronnie G.; van der Werf, Kees; Reinhardt, Dieter P.; Daamen, Willeke; Bennink, Martin L.; Dijkstra, Pieter J.; van Kuppevelt, Toin H.; Feijen, Jan.

In: Biomaterials, Vol. 30, No. 13, 2009, p. 2425-2432.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Microscale mechanical properties of single elastic fibers

T2 - The role of fibrillin-microfibrils

AU - Koenders, Mieke M.J.F.

AU - Yang, Lanti

AU - Wismans, Ronnie G.

AU - van der Werf, Kees

AU - Reinhardt, Dieter P.

AU - Daamen, Willeke

AU - Bennink, Martin L.

AU - Dijkstra, Pieter J.

AU - van Kuppevelt, Toin H.

AU - Feijen, Jan

PY - 2009

Y1 - 2009

N2 - Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.

AB - Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.

KW - IR-60757

KW - METIS-262783

KW - AFM (atomic force microscopy)

KW - Elastin

KW - Mechanical properties

KW - Mechanical test

KW - Nano-indentation

U2 - 10.1016/j.biomaterials.2009.01.038

DO - 10.1016/j.biomaterials.2009.01.038

M3 - Article

VL - 30

SP - 2425

EP - 2432

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 13

ER -

Koenders MMJF, Yang L, Wismans RG, van der Werf K, Reinhardt DP, Daamen W et al. Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils. Biomaterials. 2009;30(13):2425-2432. https://doi.org/10.1016/j.biomaterials.2009.01.038