Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils

Mieke M.J.F. Koenders; Lanti Yang; Ronnie G. Wismans; Kees van der Werf; Dieter P. Reinhardt; Willeke Daamen; Martin L. Bennink; Pieter J. Dijkstra; Toin H. van Kuppevelt; Jan Feijen

doi:10.1016/j.biomaterials.2009.01.038

Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils

Mieke M.J.F. Koenders, Lanti Yang, Ronnie G. Wismans, Kees van der Werf, Dieter P. Reinhardt, Willeke Daamen, Martin L. Bennink, Pieter J. Dijkstra, Toin H. van Kuppevelt, Jan Feijen

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Abstract

Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.

Original language	English
Pages (from-to)	2425-2432
Journal	Biomaterials
Volume	30
Issue number	13
DOIs	https://doi.org/10.1016/j.biomaterials.2009.01.038
Publication status	Published - 2009

Keywords

IR-60757
METIS-262783
AFM (atomic force microscopy)
Elastin
Mechanical properties
Mechanical test
Nano-indentation

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10.1016/j.biomaterials.2009.01.038

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@article{23fba98e6c014fa5960f04c0f9ea9180,

title = "Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils",

abstract = "Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.",

keywords = "IR-60757, METIS-262783, AFM (atomic force microscopy), Elastin, Mechanical properties, Mechanical test, Nano-indentation",

author = "Koenders, {Mieke M.J.F.} and Lanti Yang and Wismans, {Ronnie G.} and {van der Werf}, Kees and Reinhardt, {Dieter P.} and Willeke Daamen and Bennink, {Martin L.} and Dijkstra, {Pieter J.} and {van Kuppevelt}, {Toin H.} and Jan Feijen",

year = "2009",

doi = "10.1016/j.biomaterials.2009.01.038",

language = "English",

volume = "30",

pages = "2425--2432",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd",

number = "13",

}

TY - JOUR

T1 - Microscale mechanical properties of single elastic fibers

T2 - The role of fibrillin-microfibrils

AU - Koenders, Mieke M.J.F.

AU - Yang, Lanti

AU - Wismans, Ronnie G.

AU - van der Werf, Kees

AU - Reinhardt, Dieter P.

AU - Daamen, Willeke

AU - Bennink, Martin L.

AU - Dijkstra, Pieter J.

AU - van Kuppevelt, Toin H.

AU - Feijen, Jan

PY - 2009

Y1 - 2009

N2 - Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.

AB - Micromechanical properties of single elastic fibers and fibrillin-microfibrils, isolated from equine ligamentum nuchae using chemical and enzymatic methods, were determined with atomic force microscopy (AFM). Young's moduli of single elastic fibers immersed in water, devoid of or containing fibrillin-microfibrils, were determined using bending tests. Bending freely suspended elastic fibers on a micro-channeled substrate by a tip-less AFM cantilever generated a force versus displacement curve from which Young's moduli were calculated. For single elastic fibers, Young's moduli in the range of 0.3-1.5 MPa were determined, values not significantly affected by the presence of fibrillin-microfibrils. To further understand the role of fibrillin-microfibrils in vertebrate elastic fibers, layers of fibrillin-microfibrils were subjected to nano-indentation tests. From the slope of the force versus indentation curves, Young's moduli ranging between 0.56 and 0.74 MPa were calculated. The results suggest that fibrillin-microfibrils are not essential for the mechanical properties of single vertebrate elastic fibers.

KW - IR-60757

KW - METIS-262783

KW - AFM (atomic force microscopy)

KW - Elastin

KW - Mechanical properties

KW - Mechanical test

KW - Nano-indentation

U2 - 10.1016/j.biomaterials.2009.01.038

DO - 10.1016/j.biomaterials.2009.01.038

M3 - Article

SN - 0142-9612

VL - 30

SP - 2425

EP - 2432

JO - Biomaterials

JF - Biomaterials

IS - 13

ER -

Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils

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Keywords

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