Microspheres for protein delivery prepared from amphiphilic multiblock copolymers: 2. modulation of release rate

J.M. Bezemer, R. Radersma, D.W. Grijpma, P.J. Dijkstra, C.A. van Blitterswijk, J. Feijen

Research output: Contribution to journalArticleAcademicpeer-review

90 Citations (Scopus)

Abstract

Amphiphilic multiblock copolymers, based on hydrophilic poly(ethylene glycol) (PEG) blocks and hydrophobic poly(butylene terephthalate) (PBT) blocks were used as matrix material for protein-loaded microspheres. The efficiency of lysozyme entrapment by a double emulsion method was found to depend on the swelling behavior of the polymers in water and decreased from 100% for polymers with a degree of swelling of less than 1.8 to 11% for PEG–PBT copolymers with a degree of swelling of 3.6. The particle size could be controlled by varying the concentration of the polymer solution used in the microsphere preparation. An increase in the polymer concentration resulted in a proportional increase in the particle size. The in vitro release profiles of the encapsulated model protein lysozyme could be precisely tailored by variation of the copolymer composition and the size of the microspheres. Both a slow continuous release of lysozyme, and a fast release which was completed within a few days could be obtained. The release behavior, attributed to a combination of diffusion and polymer degradation, could be described by a previously developed model.
Original languageEnglish
Pages (from-to)249-260
JournalJournal of controlled release
Volume67
Issue number2-3
DOIs
Publication statusPublished - 2000

Keywords

  • Swelling
  • Protein
  • Microsphere
  • Controlled release
  • Block copolymer

Fingerprint Dive into the research topics of 'Microspheres for protein delivery prepared from amphiphilic multiblock copolymers: 2. modulation of release rate'. Together they form a unique fingerprint.

Cite this