We present a microtiter plate-sized standalone chip holder for precise control of physiological conditions inside closed microfluidic cell culture systems, made from gas-impermeable materials. Specifically, we demonstrate the suitability of the holder to support cell growth in a glass chip, to allow time-lapse imaging of live cells and the creation of a hypoxic environment, all relevant for applications in regenerative medicine research.
Harink, B., le Gac, S., Baiao Barata, D., van Blitterswijk, C., & Habibovic, P. (2014). Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices. Lab on a chip, 14(11), 1816-1820. https://doi.org/10.1039/C4LC00190G