TY - JOUR
T1 - MIFlowCyt-EV
T2 - a framework for standardized reporting of extracellular vesicle flow cytometry experiments
AU - Welsh, Joshua A.
AU - Van Der Pol, Edwin
AU - Arkesteijn, Ger J.A.
AU - Bremer, Michel
AU - Brisson, Alain
AU - Coumans, Frank
AU - Dignat-George, Françoise
AU - Duggan, Erika
AU - Ghiran, Ionita
AU - Giebel, Bernd
AU - Görgens, André
AU - Hendrix, An
AU - Lacroix, Romaric
AU - Lannigan, Joanne
AU - Libregts, Sten F.W.M.
AU - Lozano-Andrés, Estefanía
AU - Morales-Kastresana, Aizea
AU - Robert, Stephane
AU - De Rond, Leonie
AU - Tertel, Tobias
AU - Tigges, John
AU - De Wever, Olivier
AU - Yan, Xiaomei
AU - Nieuwland, Rienk
AU - Wauben, Marca H.M.
AU - Nolan, John P.
AU - Jones, Jennifer C.
N1 - Funding Information:
Supported by the Foundation for the National Institutes of Health [Pamela Cafritz Renal Cell Carcinoma Award];National Cancer Institute [1ZIA-BC011502]; National Institutes of Health [NIH UG3 TR002881]; Netherlands Organisation for Scientific Research [STW perspectief CANCER-ID 14195]; ISAC [Mary Lou Ingram Scholar]; Netherlands Organisation for Scientific Research [VENI 15924]; Netherlands Organisation for Scientific Research [VENI 13681]; ISAC [Marylou Ingram Scholar]; FWO Fund for Scientific Research; NIH [U01-126497; U01-OD-019750; R01 CA218500; R01 HL1266497; NIH UG3 TR002881]; Prostate Cancer Foundation [YIA]. JAW is an International Society for Advancement of Cytometry (ISAC) Marylou Ingram Scholar 2019-2023. RN, FC, EvdP, LdR acknowledge funding from the Netherlands Organisation for Scientific Research-Domain Applied and Engineering Sciences (NWO-TTW), research programmes VENI 13681 (FC) and VENI 15924 (EvdP) and STW perspectief CANCER-ID 14195 (LdR). IG acknowledges NIH-U01-OD-019750; NIH-R01 CA218500; NIH U01-HL126497; NIH UG3 TR002881. AG is an International Society for Advancement of Cytometry (ISAC) Marylou Ingram Scholar 2019-2023. AH and ODW are supported by Fund for Scientific Research (FWO), Foundation against Cancer (STK), Stand up to Cancer (KOTK) and Special Research Fund Ghent University (BOF). SFWML was supported by the Dutch Technology Foundation STW (Perspectief Program Cancer ID, project 14191), which is part of the Netherlands Organization for Scientific Research (NWO) and which is partly funded by the Ministry of Economic Affairs. ELA is supported by the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement No. 722148 (TRAIN-EV). JN acknowledges NIH R01 EB003824, UH3 TR000931; UH3 TR000891; UH3 TR000903. JCJ, JAW and AMK were supported by the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute and Center for Cancer Research. JCJ acknowledges NIH ZIA BC011502, NIH ZIA BC011503, NIH U01 HL126497, NIH R01 CA218500, NIH UG3 TR002881 and the Prostate Cancer Foundation.
Publisher Copyright:
© 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.
PY - 2020/2/3
Y1 - 2020/2/3
N2 - Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field’s lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratories and to support the development of new instruments and assays for improved measurement of EVs.
AB - Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field’s lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratories and to support the development of new instruments and assays for improved measurement of EVs.
KW - Extracellular vesicles
KW - flow cytometry
KW - framework
KW - reporting
KW - standardization
UR - http://www.scopus.com/inward/record.url?scp=85079217359&partnerID=8YFLogxK
U2 - 10.1080/20013078.2020.1713526
DO - 10.1080/20013078.2020.1713526
M3 - Article
AN - SCOPUS:85079217359
SN - 2001-3078
VL - 9
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
IS - 1
M1 - 1713526
ER -