Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor

L. Munoz, N.G. Dimov, G. Carot-Sans, W.P. Bula, A. Guerrero, Johannes G.E. Gardeniers

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
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Abstract

Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an “artificial gland”, i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered
Original languageEnglish
Article numbere47751
Pages (from-to)-
JournalPLoS ONE
Volume7
Issue number11
DOIs
Publication statusPublished - 14 Nov 2012

Keywords

  • METIS-290463
  • IR-84808

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