Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor

L. Munoz, N.G. Dimov, G. Carot-Sans, W.P. Bula, A. Guerrero, Johannes G.E. Gardeniers

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
67 Downloads (Pure)

Abstract

Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an “artificial gland”, i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered
Original languageEnglish
Article numbere47751
Pages (from-to)-
JournalPLoS ONE
Volume7
Issue number11
DOIs
Publication statusPublished - 14 Nov 2012

Fingerprint

insect communication
Pheromones
Transferases
transferases
pheromones
Insects
alcohols
Communication
Alcohols
Microfluidics
Spodoptera
evaporators
Spodoptera littoralis
Moths
wind tunnels
Biosynthesis
virgin females
Depolarization
Evaporators
Wind tunnels

Keywords

  • METIS-290463
  • IR-84808

Cite this

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title = "Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor",
abstract = "Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an “artificial gland”, i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40{\%} relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered",
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Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor. / Munoz, L.; Dimov, N.G.; Carot-Sans, G.; Bula, W.P.; Guerrero, A.; Gardeniers, Johannes G.E.

In: PLoS ONE, Vol. 7, No. 11, e47751, 14.11.2012, p. -.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor

AU - Munoz, L.

AU - Dimov, N.G.

AU - Carot-Sans, G.

AU - Bula, W.P.

AU - Guerrero, A.

AU - Gardeniers, Johannes G.E.

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AB - Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an “artificial gland”, i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered

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