TY - JOUR
T1 - Miniaturized Control of Acidity in Multiplexed Microreactors
AU - Balahkrishnan, Divya
AU - El Maiss, Janwa
AU - Olthuis, Wouter
AU - Pascual-García, César
N1 - Funding Information:
This research work was financed by the FNR ATTRACT project 5718158 NANOpH, and from the program H2020 Future Emerging Technlogies of the European Comission under the grant No 862539- Electromed-FET-Open. We would like to thank Mathieu Gerard for his contribution in the cover design by providing us the technical drawings of the sensors.
Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
PY - 2023/2/28
Y1 - 2023/2/28
N2 - The control of acidity drives the assembly of biopolymers that are essential for a wide range of applications. Its miniaturization can increase the speed and the possibilities of combinatorial throughput for their manipulation, similar to the way that the miniaturization of transistors allows logical operations in microelectronics with a high throughput. Here, we present a device containing multiplexed microreactors, each one enabling independent electrochemical control of acidity in ∼2.5 nL volumes, with a large acidity range from pH 3 to 7 and an accuracy of at least 0.4 pH units. The attained pH within each microreactor (with footprints of ∼0.3 mm2 for each spot) was kept constant for long retention times (∼10 min) and over repeated cycles of >100. The acidity is driven by redox proton exchange reactions, which can be driven at different rates influencing the efficiency of the device in order to achieve more charge exchange (larger acidity range) or better reversibility. The achieved performance in acidity control, miniaturization, and the possibility to multiplex paves the way for the control of combinatorial chemistry through pH- and acidity controlled reactions.
AB - The control of acidity drives the assembly of biopolymers that are essential for a wide range of applications. Its miniaturization can increase the speed and the possibilities of combinatorial throughput for their manipulation, similar to the way that the miniaturization of transistors allows logical operations in microelectronics with a high throughput. Here, we present a device containing multiplexed microreactors, each one enabling independent electrochemical control of acidity in ∼2.5 nL volumes, with a large acidity range from pH 3 to 7 and an accuracy of at least 0.4 pH units. The attained pH within each microreactor (with footprints of ∼0.3 mm2 for each spot) was kept constant for long retention times (∼10 min) and over repeated cycles of >100. The acidity is driven by redox proton exchange reactions, which can be driven at different rates influencing the efficiency of the device in order to achieve more charge exchange (larger acidity range) or better reversibility. The achieved performance in acidity control, miniaturization, and the possibility to multiplex paves the way for the control of combinatorial chemistry through pH- and acidity controlled reactions.
U2 - 10.1021/acsomega.2c06897
DO - 10.1021/acsomega.2c06897
M3 - Article
SN - 2470-1343
VL - 8
SP - 7587
EP - 7594
JO - ACS Omega
JF - ACS Omega
IS - 8
M1 - 8
ER -