Abstract
Wavefront shaping is increasingly being used in modern microscopy to obtain distortion-free, highresolution images deep inside inhomogeneous media. Wavefront shaping methods typically rely on the presence of a ‘guidestar’ in order to find the optimal wavefront to mitigate the scattering of light. However, this condition cannot be satisfied in most biomedical applications. Here, we introduce a novel, guidestar-free wavefront shaping method in which the optimal wavefront is computed using a digital model of the sample. The refractive index model of the sample, that serves as the input for the computation, is constructed in-situ by the microscope itself. In a proof of principle imaging experiment, we demonstrate a large improvement in the two-photon fluorescence signal through a diffuse medium, outperforming the state-of-the-art wavefront shaping techniques by a factor of 21.
Original language | English |
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Publisher | ArXiv.org |
Number of pages | 9 |
Publication status | Published - 14 Feb 2020 |
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Supplementary Data: Model-based wavefront shaping microscopy
Thendiyammal, A. (Creator), Vellekoop, I. M. (Creator), Osnabrugge, G. (Creator) & Knop, T. (Creator), 4TU.Centre for Research Data, 31 Mar 2020
DOI: 10.4121/uuid:06083749-0577-49d1-b1a5-8ce38f50465a
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