Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes

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    Abstract

    Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.
    Original languageUndefined
    Pages (from-to)1580-1590
    Number of pages11
    JournalIEEE transactions on biomedical engineering
    Volume49
    Issue number11
    DOIs
    Publication statusPublished - 2002

    Keywords

    • METIS-207679
    • IR-43903
    • Action potentials
    • Finite element modeling
    • Multi-electrode arrays
    • Extracellular stimulation
    • neuron-electrode contact
    • voltage-sensitive channel densities

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