Modeling mechanical signals on the surface of µCT and CAD based rapid prototype scaffold models to predict (early stage) tissue development

W.J. Hendrikson, Clemens van Blitterswijk, Nicolaas Jacobus Joseph Verdonschot, Lorenzo Moroni, Jeroen Rouwkema

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

In the field of tissue engineering, mechano-regulation theories have been applied to help predict tissue development in tissue engineering scaffolds in the past. For this, finite element models (FEMs) were used to predict the distribution of strains within a scaffold. However, the strains reported in these studies are volumetric strains of the material or strains developed in the extracellular matrix occupying the pore space. The initial phase of cell attachment and growth on the biomaterial surface has thus far been neglected. In this study, we present a model that determines the magnitude of biomechanical signals on the biomaterial surface, enabling us to predict cell differentiation stimulus values at this initial stage. Results showed that magnitudes of the 2D strain—termed surface strain—were lower when compared to the 3D volumetric strain or the conventional octahedral shear strain as used in current mechano-regulation theories. Results of both µCT and CAD derived FEMs from the same scaffold were compared. Strain and fluid shear stress distributions, and subsequently the cell differentiation stimulus, were highly dependent on the pore shape. CAD models were not able to capture the distributions seen in the µCT FEM. The calculated mechanical stimuli could be combined with current mechanobiological models resulting in a tool to predict cell differentiation in the initial phase of tissue engineering. Although experimental data is still necessary to properly link mechanical signals to cell behavior in this specific setting, this model is an important step towards optimizing scaffold architecture and/or stimulation regimes.
Original languageEnglish
Pages (from-to)1864-1875
Number of pages12
JournalBiotechnology and bioengineering
Volume111
Issue number9
DOIs
Publication statusPublished - 13 May 2014

Fingerprint

Tissue Engineering
Scaffolds
Cell Differentiation
Computer aided design
Biocompatible Materials
Tissue
Tissue Scaffolds
Tissue engineering
Extracellular Matrix
Biomaterials
Growth
Shear strain
Scaffolds (biology)
Stress concentration
Shear stress
Fluids

Keywords

  • IR-90697
  • METIS-303579

Cite this

@article{728517a521e94efdb2b17e17d1bc102f,
title = "Modeling mechanical signals on the surface of µCT and CAD based rapid prototype scaffold models to predict (early stage) tissue development",
abstract = "In the field of tissue engineering, mechano-regulation theories have been applied to help predict tissue development in tissue engineering scaffolds in the past. For this, finite element models (FEMs) were used to predict the distribution of strains within a scaffold. However, the strains reported in these studies are volumetric strains of the material or strains developed in the extracellular matrix occupying the pore space. The initial phase of cell attachment and growth on the biomaterial surface has thus far been neglected. In this study, we present a model that determines the magnitude of biomechanical signals on the biomaterial surface, enabling us to predict cell differentiation stimulus values at this initial stage. Results showed that magnitudes of the 2D strain—termed surface strain—were lower when compared to the 3D volumetric strain or the conventional octahedral shear strain as used in current mechano-regulation theories. Results of both µCT and CAD derived FEMs from the same scaffold were compared. Strain and fluid shear stress distributions, and subsequently the cell differentiation stimulus, were highly dependent on the pore shape. CAD models were not able to capture the distributions seen in the µCT FEM. The calculated mechanical stimuli could be combined with current mechanobiological models resulting in a tool to predict cell differentiation in the initial phase of tissue engineering. Although experimental data is still necessary to properly link mechanical signals to cell behavior in this specific setting, this model is an important step towards optimizing scaffold architecture and/or stimulation regimes.",
keywords = "IR-90697, METIS-303579",
author = "W.J. Hendrikson and {van Blitterswijk}, Clemens and Verdonschot, {Nicolaas Jacobus Joseph} and Lorenzo Moroni and Jeroen Rouwkema",
note = "Early View (Online Version of Record published before inclusion in an issue)",
year = "2014",
month = "5",
day = "13",
doi = "10.1002/bit.25231",
language = "English",
volume = "111",
pages = "1864--1875",
journal = "Biotechnology and bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "9",

}

Modeling mechanical signals on the surface of µCT and CAD based rapid prototype scaffold models to predict (early stage) tissue development. / Hendrikson, W.J.; van Blitterswijk, Clemens; Verdonschot, Nicolaas Jacobus Joseph; Moroni, Lorenzo; Rouwkema, Jeroen.

In: Biotechnology and bioengineering, Vol. 111, No. 9, 13.05.2014, p. 1864-1875.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Modeling mechanical signals on the surface of µCT and CAD based rapid prototype scaffold models to predict (early stage) tissue development

AU - Hendrikson, W.J.

AU - van Blitterswijk, Clemens

AU - Verdonschot, Nicolaas Jacobus Joseph

AU - Moroni, Lorenzo

AU - Rouwkema, Jeroen

N1 - Early View (Online Version of Record published before inclusion in an issue)

PY - 2014/5/13

Y1 - 2014/5/13

N2 - In the field of tissue engineering, mechano-regulation theories have been applied to help predict tissue development in tissue engineering scaffolds in the past. For this, finite element models (FEMs) were used to predict the distribution of strains within a scaffold. However, the strains reported in these studies are volumetric strains of the material or strains developed in the extracellular matrix occupying the pore space. The initial phase of cell attachment and growth on the biomaterial surface has thus far been neglected. In this study, we present a model that determines the magnitude of biomechanical signals on the biomaterial surface, enabling us to predict cell differentiation stimulus values at this initial stage. Results showed that magnitudes of the 2D strain—termed surface strain—were lower when compared to the 3D volumetric strain or the conventional octahedral shear strain as used in current mechano-regulation theories. Results of both µCT and CAD derived FEMs from the same scaffold were compared. Strain and fluid shear stress distributions, and subsequently the cell differentiation stimulus, were highly dependent on the pore shape. CAD models were not able to capture the distributions seen in the µCT FEM. The calculated mechanical stimuli could be combined with current mechanobiological models resulting in a tool to predict cell differentiation in the initial phase of tissue engineering. Although experimental data is still necessary to properly link mechanical signals to cell behavior in this specific setting, this model is an important step towards optimizing scaffold architecture and/or stimulation regimes.

AB - In the field of tissue engineering, mechano-regulation theories have been applied to help predict tissue development in tissue engineering scaffolds in the past. For this, finite element models (FEMs) were used to predict the distribution of strains within a scaffold. However, the strains reported in these studies are volumetric strains of the material or strains developed in the extracellular matrix occupying the pore space. The initial phase of cell attachment and growth on the biomaterial surface has thus far been neglected. In this study, we present a model that determines the magnitude of biomechanical signals on the biomaterial surface, enabling us to predict cell differentiation stimulus values at this initial stage. Results showed that magnitudes of the 2D strain—termed surface strain—were lower when compared to the 3D volumetric strain or the conventional octahedral shear strain as used in current mechano-regulation theories. Results of both µCT and CAD derived FEMs from the same scaffold were compared. Strain and fluid shear stress distributions, and subsequently the cell differentiation stimulus, were highly dependent on the pore shape. CAD models were not able to capture the distributions seen in the µCT FEM. The calculated mechanical stimuli could be combined with current mechanobiological models resulting in a tool to predict cell differentiation in the initial phase of tissue engineering. Although experimental data is still necessary to properly link mechanical signals to cell behavior in this specific setting, this model is an important step towards optimizing scaffold architecture and/or stimulation regimes.

KW - IR-90697

KW - METIS-303579

U2 - 10.1002/bit.25231

DO - 10.1002/bit.25231

M3 - Article

VL - 111

SP - 1864

EP - 1875

JO - Biotechnology and bioengineering

JF - Biotechnology and bioengineering

SN - 0006-3592

IS - 9

ER -