TY - JOUR
T1 - Modeling single cell antibody excretion on a biosensor
AU - Stojanovic, Ivan
AU - Baumgartner, W.
AU - van der Velden, T.J.G.
AU - Terstappen, Leonardus Wendelinus Mathias Marie
AU - Schasfoort, Richardus B.M.
PY - 2016/3/31
Y1 - 2016/3/31
N2 - We simulated, using Comsol Multiphysics, the excretion of antibodies by single hybridoma cells and their subsequent binding on a surface plasmon resonance imaging (SPRi) sensor. The purpose was to confirm that SPRi is suitable to accurately quantify antibody (anti-EpCAM) excretion. The model showed that antibody loss by diffusion away from the sensor was less than 1%. Unexpectedly, more than 99% of the excreted antibodies were captured on the sensor. These data prove the remarkable phenomenon that the SPRi output of cellular antibody excretion and its subsequent binding, performed under the conditions described here, is directly usable for quantification of single cell antibody production rates.
AB - We simulated, using Comsol Multiphysics, the excretion of antibodies by single hybridoma cells and their subsequent binding on a surface plasmon resonance imaging (SPRi) sensor. The purpose was to confirm that SPRi is suitable to accurately quantify antibody (anti-EpCAM) excretion. The model showed that antibody loss by diffusion away from the sensor was less than 1%. Unexpectedly, more than 99% of the excreted antibodies were captured on the sensor. These data prove the remarkable phenomenon that the SPRi output of cellular antibody excretion and its subsequent binding, performed under the conditions described here, is directly usable for quantification of single cell antibody production rates.
KW - METIS-318828
KW - IR-103822
U2 - 10.1016/j.ab.2016.03.018
DO - 10.1016/j.ab.2016.03.018
M3 - Article
VL - 504
SP - 1
EP - 3
JO - Analytical biochemistry
JF - Analytical biochemistry
SN - 0003-2697
ER -