Modeling single cell antibody excretion on a biosensor

Ivan Stojanovic, W. Baumgartner, T.J.G. van der Velden, Leonardus Wendelinus Mathias Marie Terstappen, Richardus B.M. Schasfoort

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

We simulated, using Comsol Multiphysics, the excretion of antibodies by single hybridoma cells and their subsequent binding on a surface plasmon resonance imaging (SPRi) sensor. The purpose was to confirm that SPRi is suitable to accurately quantify antibody (anti-EpCAM) excretion. The model showed that antibody loss by diffusion away from the sensor was less than 1%. Unexpectedly, more than 99% of the excreted antibodies were captured on the sensor. These data prove the remarkable phenomenon that the SPRi output of cellular antibody excretion and its subsequent binding, performed under the conditions described here, is directly usable for quantification of single cell antibody production rates.
Original languageEnglish
Pages (from-to)1-3
JournalAnalytical biochemistry
Volume504
DOIs
Publication statusPublished - 31 Mar 2016

Keywords

  • METIS-318828
  • IR-103822

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