Modification of mechanical environment to control vascular organization within developing chicken embryo

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Abstract

INTRODUCTION: Vascular tree formation and their network organization has previously been shown to be sensitive to the mechanical environment in vitro, yet the specific relationship between the mechanical factors such as geometry, shape, pressure, flow characteristics and vascular organization in vivo is not well understood. The present study aimed to investigate the effect of yolk shape on vascular network organization, within developing chicken embryos cultured in containers with different geometries, resulting in a different mechanical environment of the egg yolk. Using both laser speckle contrast (LSCI) and laser doppler perfusion imaging (LDPI) techniques, the spatiotemporal changes of heart rate and flow velocity in the vascular networks within developing chickenembryos were determined.METHODS: PDMS based artificial egg shell systems: 3D geometric (cube, cylinder and triangular prism shaped) containers based on oxygen permeable thin polydimethylsiloxane (PDMS) membranes were assembled using soft lithographic templates. 3D printed poly(lactic acid) frames were used as mechanical support. Fertilized white leghorn chicken eggs were incubated with 38°C and 65%humidity under regular rotation. After 3-days of incubation, chicken embryos were transferred to the artificial geometric culture systems as shown in the figure.RESULTS & DISCUSSION: Results showed that the heart beat rate (figure -right) and vascular network density were influenced by changing the local mechanical environment of egg yolk. Further, LSCI revealed changes in the perfusion rates within the chick vasculature. Changes in vascular organization with respect to differences in the local microenvironment of the yolk were observed.CONCLUSIONS: With the ultimate goal to understand in vivo vascular organization, fluid flow and growth factor gradient patterns adjacent to the developing chicken blood vessels will next be introduced in the PDMS systems. This 3D integrated platform offers the possibility to evaluate the effect of multiple signals towards vascular organization in a single system.Figure1: (left) chicken embryos exposed to different mechanical environments and (right) variations in perfusion rates of chick vasculature subjected to different mechanical environmentsACKNOWLEDGEMENTS: This work is supported by an ERC Consolidator Grant under grant agreement no 724469 and NWO Dutch funding with project number 14538.
Original languageEnglish
Pages1259 - 1259
Number of pages1
Publication statusPublished - 27 May 2019
EventTERMIS European Chapter Meeting 2019: Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation - Rodos Palace, Rhodes, Greece
Duration: 27 May 201931 May 2019
https://www.termis.org/eu2019/

Conference

ConferenceTERMIS European Chapter Meeting 2019
Abbreviated titleTERMIS EU 2019
CountryGreece
CityRhodes
Period27/05/1931/05/19
Internet address

Fingerprint

Blood Vessels
Chickens
Embryonic Structures
Egg Yolk
Organizations
Lasers
Perfusion
Heart Rate
Egg Shell
Perfusion Imaging
Organized Financing
Humidity
Eggs
Intercellular Signaling Peptides and Proteins
Oxygen
Pressure
Membranes
baysilon

Keywords

  • yolk shape
  • vascular organization
  • LSCI
  • Chicken embryo
  • perfusion imaging
  • mechanical stimuli

Cite this

Padmanaban, P., Chizari, A., Steenbergen, W., & Rouwkema, J. (2019). Modification of mechanical environment to control vascular organization within developing chicken embryo. 1259 - 1259. Abstract from TERMIS European Chapter Meeting 2019, Rhodes, Greece.
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abstract = "INTRODUCTION: Vascular tree formation and their network organization has previously been shown to be sensitive to the mechanical environment in vitro, yet the specific relationship between the mechanical factors such as geometry, shape, pressure, flow characteristics and vascular organization in vivo is not well understood. The present study aimed to investigate the effect of yolk shape on vascular network organization, within developing chicken embryos cultured in containers with different geometries, resulting in a different mechanical environment of the egg yolk. Using both laser speckle contrast (LSCI) and laser doppler perfusion imaging (LDPI) techniques, the spatiotemporal changes of heart rate and flow velocity in the vascular networks within developing chickenembryos were determined.METHODS: PDMS based artificial egg shell systems: 3D geometric (cube, cylinder and triangular prism shaped) containers based on oxygen permeable thin polydimethylsiloxane (PDMS) membranes were assembled using soft lithographic templates. 3D printed poly(lactic acid) frames were used as mechanical support. Fertilized white leghorn chicken eggs were incubated with 38°C and 65{\%}humidity under regular rotation. After 3-days of incubation, chicken embryos were transferred to the artificial geometric culture systems as shown in the figure.RESULTS & DISCUSSION: Results showed that the heart beat rate (figure -right) and vascular network density were influenced by changing the local mechanical environment of egg yolk. Further, LSCI revealed changes in the perfusion rates within the chick vasculature. Changes in vascular organization with respect to differences in the local microenvironment of the yolk were observed.CONCLUSIONS: With the ultimate goal to understand in vivo vascular organization, fluid flow and growth factor gradient patterns adjacent to the developing chicken blood vessels will next be introduced in the PDMS systems. This 3D integrated platform offers the possibility to evaluate the effect of multiple signals towards vascular organization in a single system.Figure1: (left) chicken embryos exposed to different mechanical environments and (right) variations in perfusion rates of chick vasculature subjected to different mechanical environmentsACKNOWLEDGEMENTS: This work is supported by an ERC Consolidator Grant under grant agreement no 724469 and NWO Dutch funding with project number 14538.",
keywords = "yolk shape, vascular organization, LSCI, Chicken embryo, perfusion imaging, mechanical stimuli",
author = "Prasanna Padmanaban and Ata Chizari and Wiendelt Steenbergen and J. Rouwkema",
year = "2019",
month = "5",
day = "27",
language = "English",
pages = "1259 -- 1259",
note = "TERMIS European Chapter Meeting 2019 : Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, TERMIS EU 2019 ; Conference date: 27-05-2019 Through 31-05-2019",
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Padmanaban, P, Chizari, A, Steenbergen, W & Rouwkema, J 2019, 'Modification of mechanical environment to control vascular organization within developing chicken embryo' TERMIS European Chapter Meeting 2019, Rhodes, Greece, 27/05/19 - 31/05/19, pp. 1259 - 1259.

Modification of mechanical environment to control vascular organization within developing chicken embryo. / Padmanaban, Prasanna ; Chizari, Ata ; Steenbergen, Wiendelt ; Rouwkema, J.

2019. 1259 - 1259 Abstract from TERMIS European Chapter Meeting 2019, Rhodes, Greece.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Modification of mechanical environment to control vascular organization within developing chicken embryo

AU - Padmanaban, Prasanna

AU - Chizari, Ata

AU - Steenbergen, Wiendelt

AU - Rouwkema, J.

PY - 2019/5/27

Y1 - 2019/5/27

N2 - INTRODUCTION: Vascular tree formation and their network organization has previously been shown to be sensitive to the mechanical environment in vitro, yet the specific relationship between the mechanical factors such as geometry, shape, pressure, flow characteristics and vascular organization in vivo is not well understood. The present study aimed to investigate the effect of yolk shape on vascular network organization, within developing chicken embryos cultured in containers with different geometries, resulting in a different mechanical environment of the egg yolk. Using both laser speckle contrast (LSCI) and laser doppler perfusion imaging (LDPI) techniques, the spatiotemporal changes of heart rate and flow velocity in the vascular networks within developing chickenembryos were determined.METHODS: PDMS based artificial egg shell systems: 3D geometric (cube, cylinder and triangular prism shaped) containers based on oxygen permeable thin polydimethylsiloxane (PDMS) membranes were assembled using soft lithographic templates. 3D printed poly(lactic acid) frames were used as mechanical support. Fertilized white leghorn chicken eggs were incubated with 38°C and 65%humidity under regular rotation. After 3-days of incubation, chicken embryos were transferred to the artificial geometric culture systems as shown in the figure.RESULTS & DISCUSSION: Results showed that the heart beat rate (figure -right) and vascular network density were influenced by changing the local mechanical environment of egg yolk. Further, LSCI revealed changes in the perfusion rates within the chick vasculature. Changes in vascular organization with respect to differences in the local microenvironment of the yolk were observed.CONCLUSIONS: With the ultimate goal to understand in vivo vascular organization, fluid flow and growth factor gradient patterns adjacent to the developing chicken blood vessels will next be introduced in the PDMS systems. This 3D integrated platform offers the possibility to evaluate the effect of multiple signals towards vascular organization in a single system.Figure1: (left) chicken embryos exposed to different mechanical environments and (right) variations in perfusion rates of chick vasculature subjected to different mechanical environmentsACKNOWLEDGEMENTS: This work is supported by an ERC Consolidator Grant under grant agreement no 724469 and NWO Dutch funding with project number 14538.

AB - INTRODUCTION: Vascular tree formation and their network organization has previously been shown to be sensitive to the mechanical environment in vitro, yet the specific relationship between the mechanical factors such as geometry, shape, pressure, flow characteristics and vascular organization in vivo is not well understood. The present study aimed to investigate the effect of yolk shape on vascular network organization, within developing chicken embryos cultured in containers with different geometries, resulting in a different mechanical environment of the egg yolk. Using both laser speckle contrast (LSCI) and laser doppler perfusion imaging (LDPI) techniques, the spatiotemporal changes of heart rate and flow velocity in the vascular networks within developing chickenembryos were determined.METHODS: PDMS based artificial egg shell systems: 3D geometric (cube, cylinder and triangular prism shaped) containers based on oxygen permeable thin polydimethylsiloxane (PDMS) membranes were assembled using soft lithographic templates. 3D printed poly(lactic acid) frames were used as mechanical support. Fertilized white leghorn chicken eggs were incubated with 38°C and 65%humidity under regular rotation. After 3-days of incubation, chicken embryos were transferred to the artificial geometric culture systems as shown in the figure.RESULTS & DISCUSSION: Results showed that the heart beat rate (figure -right) and vascular network density were influenced by changing the local mechanical environment of egg yolk. Further, LSCI revealed changes in the perfusion rates within the chick vasculature. Changes in vascular organization with respect to differences in the local microenvironment of the yolk were observed.CONCLUSIONS: With the ultimate goal to understand in vivo vascular organization, fluid flow and growth factor gradient patterns adjacent to the developing chicken blood vessels will next be introduced in the PDMS systems. This 3D integrated platform offers the possibility to evaluate the effect of multiple signals towards vascular organization in a single system.Figure1: (left) chicken embryos exposed to different mechanical environments and (right) variations in perfusion rates of chick vasculature subjected to different mechanical environmentsACKNOWLEDGEMENTS: This work is supported by an ERC Consolidator Grant under grant agreement no 724469 and NWO Dutch funding with project number 14538.

KW - yolk shape

KW - vascular organization

KW - LSCI

KW - Chicken embryo

KW - perfusion imaging

KW - mechanical stimuli

M3 - Abstract

SP - 1259

EP - 1259

ER -