Modulation-frequency encoded multi-color fluorescent DNA analysis in an optofluidic chip

Chaitanya Dongre, Jasper van Weerd, Geert A.J. Besselink, Rebecca Martinez Vazquez, Roberto Osellame, Giulio Cerullo, Rob van Weeghel, Hans H. van den Vlekkert, Hugo J.W.M. Hoekstra, Markus Pollnau

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    We introduce a principle of parallel optical processing to an optofluidic lab-on-a-chip. During electrophoretic separation, the ultra-low limit of detection achieved with our set-up allows us to record fluorescence from covalently end-labeled DNA molecules. Different sets of exclusively color-labeled DNA fragments—otherwise rendered indistinguishable by spatio-temporal coincidence—are traced back to their origin by modulation-frequency-encoded multi- avelength laser excitation, fluorescence detection with a single ultrasensitive, albeit color-blind photomultiplier, and Fourier analysis decoding. As a proof of principle, fragments obtained by multiplex ligation-dependent probe amplification from independent human genomic segments, associated with genetic predispositions to breast cancer and anemia, are simultaneously analyzed.
    Original languageEnglish
    Pages (from-to)679-683
    Number of pages5
    JournalLab on a chip
    Issue number4
    Publication statusPublished - Jan 2011



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