Molecular printboards as general platforms for protein immobilization

Manon Julia Wilhelmina Ludden

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

38 Downloads (Pure)

Abstract

This thesis describes the selective attachment of proteins to β-cyclodextrin (βCD) self-assembled monolayers (SAMs), termed molecular printboards through multivalent orthogonal interactions. It is shown that the molecular printboards allow different assembly pathways for the build-up of (complex) bionanostructures. In the assembly of these bionanostructures, control over stability, stoichiometry of binding, and orientation is achieved. A monovalent supramolecular blocking agent can be applied to prevent nonspecific immobilization of proteins to the molecular printboard, while the specific attachment of proteins via multivalent interactions is still possible.
Original languageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Huskens, Jurriaan , Supervisor
  • Reinhoudt, David Nicolaas, Supervisor
Award date21 Sep 2007
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-2521-3
Publication statusPublished - 21 Sep 2007

Fingerprint

Proteins
Cyclodextrins
Self assembled monolayers
Stoichiometry

Keywords

  • IR-58008

Cite this

Ludden, M. J. W. (2007). Molecular printboards as general platforms for protein immobilization. Enschede: University of Twente.
Ludden, Manon Julia Wilhelmina. / Molecular printboards as general platforms for protein immobilization. Enschede : University of Twente, 2007. 184 p.
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Ludden, MJW 2007, 'Molecular printboards as general platforms for protein immobilization', University of Twente, Enschede.

Molecular printboards as general platforms for protein immobilization. / Ludden, Manon Julia Wilhelmina.

Enschede : University of Twente, 2007. 184 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

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N2 - This thesis describes the selective attachment of proteins to β-cyclodextrin (βCD) self-assembled monolayers (SAMs), termed molecular printboards through multivalent orthogonal interactions. It is shown that the molecular printboards allow different assembly pathways for the build-up of (complex) bionanostructures. In the assembly of these bionanostructures, control over stability, stoichiometry of binding, and orientation is achieved. A monovalent supramolecular blocking agent can be applied to prevent nonspecific immobilization of proteins to the molecular printboard, while the specific attachment of proteins via multivalent interactions is still possible.

AB - This thesis describes the selective attachment of proteins to β-cyclodextrin (βCD) self-assembled monolayers (SAMs), termed molecular printboards through multivalent orthogonal interactions. It is shown that the molecular printboards allow different assembly pathways for the build-up of (complex) bionanostructures. In the assembly of these bionanostructures, control over stability, stoichiometry of binding, and orientation is achieved. A monovalent supramolecular blocking agent can be applied to prevent nonspecific immobilization of proteins to the molecular printboard, while the specific attachment of proteins via multivalent interactions is still possible.

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Ludden MJW. Molecular printboards as general platforms for protein immobilization. Enschede: University of Twente, 2007. 184 p.