Monitoring of DNA molecules in a lab on a chip with femtosecond laser written waveguides

Markus Pollnau, C. Dongre, R. Dekker, Hugo Hoekstra, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, G.A.J. Besselink, H.H. van den Vlekkert

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

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    Abstract

    Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 um, enabling high-resolution monitoring of the passage of different types of fluorescent analytes, when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. We demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled Deoxyribonucleic acid (DNA) molecules, as well as separation of different dyes, Rhodamine-6G and Rhodamine-B during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low detection limit will enable monitoring of extremely small quantities with high spatial resolution.
    Original languageEnglish
    Title of host publication39th Winter Colloquium on The Physics of Quantum Electronics 2009
    Place of PublicationCollege Station, TX, USA
    PublisherTexas A & M University
    Pages213
    Number of pages1
    Publication statusPublished - Jan 2009
    Event39th Winter Colloquium on The Physics of Quantum Electronics 2009 - Snowbird, United States
    Duration: 4 Jan 20098 Jan 2009
    Conference number: 39
    http://www.pqeconference.com/pqe2009/

    Conference

    Conference39th Winter Colloquium on The Physics of Quantum Electronics 2009
    CountryUnited States
    CitySnowbird
    Period4/01/098/01/09
    Internet address

    Keywords

    • IOMS-SNS: SENSORS

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