Monitoring of yeast cell concentration using a micromachined impedance sensor

E.E. Krommenhoek; Johannes G.E. Gardeniers; Johan G. Bomer; Albert van den Berg; X. Li; M. Ottens; L.A.M. van der Wielen; G.W.K. van Dedem; M. van Leeuwen; W.M. van Gulik; J.J. Heijnen

doi:10.1109/SENSOR.2005.1496412

Monitoring of yeast cell concentration using a micromachined impedance sensor

E.E. Krommenhoek, Johannes G.E. Gardeniers, Johan G. Bomer, Albert van den Berg, X. Li, M. Ottens, L.A.M. van der Wielen, G.W.K. van Dedem, M. van Leeuwen, W.M. van Gulik, J.J. Heijnen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

210 Downloads (Pure)

Abstract

The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.

Original language	Undefined
Title of host publication	The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)
Place of Publication	USA
Publisher	IEEE
Pages	283-286
Number of pages	4
ISBN (Print)	0-7803-8994-8
DOIs	https://doi.org/10.1109/SENSOR.2005.1496412
Publication status	Published - 5 Jun 2005
Event	13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005 - Seoul, Korea, Republic of Duration: 5 Jun 2005 → 9 Jun 2005 Conference number: 13

Publication series

Name
Publisher	IEEE
Volume	1

Conference

Conference	13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005
Abbreviated title	TRANSDUCERS
Country/Territory	Korea, Republic of
City	Seoul
Period	5/06/05 → 9/06/05

Keywords

METIS-223988
Dielectric spectroscopy
IR-51047
Microporous membrane
cell concentration
permittivity
pHEMA
EWI-18737

Access to Document

10.1109/SENSOR.2005.1496412

Cite this

Krommenhoek, E. E., Gardeniers, J. G. E., Bomer, J. G., van den Berg, A., Li, X., Ottens, M., van der Wielen, L. A. M., van Dedem, G. W. K., van Leeuwen, M., van Gulik, W. M., & Heijnen, J. J. (2005). Monitoring of yeast cell concentration using a micromachined impedance sensor. In The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005) (pp. 283-286). IEEE. https://doi.org/10.1109/SENSOR.2005.1496412

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abstract = "The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.",

keywords = "METIS-223988, Dielectric spectroscopy, IR-51047, Microporous membrane, cell concentration, permittivity, pHEMA, EWI-18737",

author = "E.E. Krommenhoek and Gardeniers, {Johannes G.E.} and Bomer, {Johan G.} and {van den Berg}, Albert and X. Li and M. Ottens and {van der Wielen}, L.A.M. and {van Dedem}, G.W.K. and {van Leeuwen}, M. and {van Gulik}, W.M. and J.J. Heijnen",

year = "2005",

month = jun,

day = "5",

doi = "10.1109/SENSOR.2005.1496412",

language = "Undefined",

isbn = "0-7803-8994-8",

publisher = "IEEE",

pages = "283--286",

booktitle = "The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)",

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note = "13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005 ; Conference date: 05-06-2005 Through 09-06-2005",

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Krommenhoek, EE, Gardeniers, JGE , Bomer, JG , van den Berg, A, Li, X, Ottens, M, van der Wielen, LAM, van Dedem, GWK, van Leeuwen, M, van Gulik, WM & Heijnen, JJ 2005, Monitoring of yeast cell concentration using a micromachined impedance sensor. in The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). IEEE, USA, pp. 283-286, 13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005, Seoul, Korea, Republic of, 5/06/05. https://doi.org/10.1109/SENSOR.2005.1496412

Monitoring of yeast cell concentration using a micromachined impedance sensor. / Krommenhoek, E.E.; Gardeniers, Johannes G.E.; Bomer, Johan G. et al.
The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). USA: IEEE, 2005. p. 283-286.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Monitoring of yeast cell concentration using a micromachined impedance sensor

AU - Krommenhoek, E.E.

AU - Gardeniers, Johannes G.E.

AU - Bomer, Johan G.

AU - van den Berg, Albert

AU - Li, X.

AU - Ottens, M.

AU - van der Wielen, L.A.M.

AU - van Dedem, G.W.K.

AU - van Leeuwen, M.

AU - van Gulik, W.M.

AU - Heijnen, J.J.

N1 - Conference code: 13

PY - 2005/6/5

Y1 - 2005/6/5

N2 - The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.

AB - The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.

KW - METIS-223988

KW - Dielectric spectroscopy

KW - IR-51047

KW - Microporous membrane

KW - cell concentration

KW - permittivity

KW - pHEMA

KW - EWI-18737

U2 - 10.1109/SENSOR.2005.1496412

DO - 10.1109/SENSOR.2005.1496412

M3 - Conference contribution

SN - 0-7803-8994-8

SP - 283

EP - 286

BT - The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)

PB - IEEE

CY - USA

T2 - 13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005

Y2 - 5 June 2005 through 9 June 2005

ER -