Monitoring of yeast cell concentration using a micromachined impedance sensor

E.E. Krommenhoek, Johannes G.E. Gardeniers, Johan G. Bomer, Albert van den Berg, X. Li, M. Ottens, L.A.M. van der Wielen, G.W.K. van Dedem, M. van Leeuwen, W.M. van Gulik, J.J. Heijnen

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

53 Downloads (Pure)

Abstract

The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.
Original languageUndefined
Title of host publicationThe 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)
Place of PublicationUSA
PublisherIEEE
Pages283-286
Number of pages4
ISBN (Print)0-7803-8994-8
DOIs
Publication statusPublished - 5 Jun 2005
Event13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005 - Seoul, Korea, Republic of
Duration: 5 Jun 20059 Jun 2005
Conference number: 13

Publication series

Name
PublisherIEEE
Volume1

Conference

Conference13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005
Abbreviated titleTRANSDUCERS
CountryKorea, Republic of
CitySeoul
Period5/06/059/06/05

Keywords

  • METIS-223988
  • Dielectric spectroscopy
  • IR-51047
  • Microporous membrane
  • cell concentration
  • permittivity
  • pHEMA
  • EWI-18737

Cite this

Krommenhoek, E. E., Gardeniers, J. G. E., Bomer, J. G., van den Berg, A., Li, X., Ottens, M., ... Heijnen, J. J. (2005). Monitoring of yeast cell concentration using a micromachined impedance sensor. In The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005) (pp. 283-286). USA: IEEE. https://doi.org/10.1109/SENSOR.2005.1496412
Krommenhoek, E.E. ; Gardeniers, Johannes G.E. ; Bomer, Johan G. ; van den Berg, Albert ; Li, X. ; Ottens, M. ; van der Wielen, L.A.M. ; van Dedem, G.W.K. ; van Leeuwen, M. ; van Gulik, W.M. ; Heijnen, J.J. / Monitoring of yeast cell concentration using a micromachined impedance sensor. The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). USA : IEEE, 2005. pp. 283-286
@inproceedings{a90d12e5d9b7424d8e01bc8ca1ccb921,
title = "Monitoring of yeast cell concentration using a micromachined impedance sensor",
abstract = "The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.",
keywords = "METIS-223988, Dielectric spectroscopy, IR-51047, Microporous membrane, cell concentration, permittivity, pHEMA, EWI-18737",
author = "E.E. Krommenhoek and Gardeniers, {Johannes G.E.} and Bomer, {Johan G.} and {van den Berg}, Albert and X. Li and M. Ottens and {van der Wielen}, L.A.M. and {van Dedem}, G.W.K. and {van Leeuwen}, M. and {van Gulik}, W.M. and J.J. Heijnen",
year = "2005",
month = "6",
day = "5",
doi = "10.1109/SENSOR.2005.1496412",
language = "Undefined",
isbn = "0-7803-8994-8",
publisher = "IEEE",
pages = "283--286",
booktitle = "The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)",
address = "United States",

}

Krommenhoek, EE, Gardeniers, JGE, Bomer, JG, van den Berg, A, Li, X, Ottens, M, van der Wielen, LAM, van Dedem, GWK, van Leeuwen, M, van Gulik, WM & Heijnen, JJ 2005, Monitoring of yeast cell concentration using a micromachined impedance sensor. in The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). IEEE, USA, pp. 283-286, 13th International Conferences on Solid-State Sensors, Actuators and Mircosystems, TRANSDUCERS 2005, Seoul, Korea, Republic of, 5/06/05. https://doi.org/10.1109/SENSOR.2005.1496412

Monitoring of yeast cell concentration using a micromachined impedance sensor. / Krommenhoek, E.E.; Gardeniers, Johannes G.E.; Bomer, Johan G.; van den Berg, Albert; Li, X.; Ottens, M.; van der Wielen, L.A.M.; van Dedem, G.W.K.; van Leeuwen, M.; van Gulik, W.M.; Heijnen, J.J.

The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). USA : IEEE, 2005. p. 283-286.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Monitoring of yeast cell concentration using a micromachined impedance sensor

AU - Krommenhoek, E.E.

AU - Gardeniers, Johannes G.E.

AU - Bomer, Johan G.

AU - van den Berg, Albert

AU - Li, X.

AU - Ottens, M.

AU - van der Wielen, L.A.M.

AU - van Dedem, G.W.K.

AU - van Leeuwen, M.

AU - van Gulik, W.M.

AU - Heijnen, J.J.

PY - 2005/6/5

Y1 - 2005/6/5

N2 - The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.

AB - The paper describes the design, modelling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in a Saccharomyces cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/l. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.

KW - METIS-223988

KW - Dielectric spectroscopy

KW - IR-51047

KW - Microporous membrane

KW - cell concentration

KW - permittivity

KW - pHEMA

KW - EWI-18737

U2 - 10.1109/SENSOR.2005.1496412

DO - 10.1109/SENSOR.2005.1496412

M3 - Conference contribution

SN - 0-7803-8994-8

SP - 283

EP - 286

BT - The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005)

PB - IEEE

CY - USA

ER -

Krommenhoek EE, Gardeniers JGE, Bomer JG, van den Berg A, Li X, Ottens M et al. Monitoring of yeast cell concentration using a micromachined impedance sensor. In The 13th International Conference on Solid-StateSensors, Actuators and Microsystems, (Transducers 2005). USA: IEEE. 2005. p. 283-286 https://doi.org/10.1109/SENSOR.2005.1496412